Electrosprayed cefazolin-loaded niosomes onto electrospun chitosan nanofibrous membrane for wound healing applications.

Autor: Mansouri M Boroujeni; Bacteriology Department, Pasteur Institute of Iran, Tehran, Iran., Barzi SM; Bacteriology Department, Pasteur Institute of Iran, Tehran, Iran., Zafari M; Bacteriology Department, Pasteur Institute of Iran, Tehran, Iran.; National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran., Chiani M; Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran., Chehrazi M; School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran., Nosrati H; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran., Shams Nosrati MS; Virology Department, Pasteur Institute of Iran, Tehran, Iran., Nayyeri S; National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran., Khodaei M; Materials Engineering Group, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan, Iran., Bonakdar S; National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran., Shafiei M; Bacteriology Department, Pasteur Institute of Iran, Tehran, Iran.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2022 Aug; Vol. 110 (8), pp. 1814-1826. Date of Electronic Publication: 2022 Feb 23.
DOI: 10.1002/jbm.b.35039
Abstrakt: Chronic wounds are among the most therapeutically challenging conditions, which are commonly followed by bacterial infection. The ideal approach to treat such injuries are synergistic infection therapy and skin tissue regeneration. In the recent decades, nanotechnology has played a critical role in eradicating bacterial infections by introducing several carriers developed for drug delivery. Moreover, advances in tissue engineering have resulted in new drug delivery systems that can improve the skin regeneration rate and quality. In this study, cefazolin-loaded niosomes were electrosprayed onto chitosan membrane for wound healing applications. For this purpose, niosomes were obtained by the thin-film hydration method; electrospinning was then conducted to fabricate nanofibrous mats. In vitro characterization of the scaffold was performed to evaluate the physicochemical and biological properties. Finally, in vivo studies were carried out to evaluate the potential use of the membrane for skin regeneration. In vitro results indicated the antibacterial properties of the membrane against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) due to the gradual release of cefazolin from niosomes. The scaffolds also showed no cell toxicity. In vivo studies also confirmed the ability of the membrane to enhance skin regeneration by improving re-epithelialization, tissue remodeling, and angiogenesis. The current study could well show the promising role of the prepared scaffold for skin regeneration and bacterial infection elimination.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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