Inflammation is associated with impairment of oxidative status, carbohydrate and lipid metabolism in type 2 diabetes complicated by non-alcoholic fatty liver disease.

Autor:	Popov SS; Department of Hospital Therapy and Endocrinology, Voronezh State Medical University Named After N.N. Burdenko, Voronezh, Russia., Kryl'skii ED; Department of Medical Biochemistry and Microbiology, Voronezh State University, Voronezh, Russia - evgenij.krylsky@yandex.ru., Shulgin KK; Department of Medical Biochemistry and Microbiology, Voronezh State University, Voronezh, Russia., Raskina EA; Department of Hospital Therapy and Endocrinology, Voronezh State Medical University Named After N.N. Burdenko, Voronezh, Russia., Popova TN; Department of Medical Biochemistry and Microbiology, Voronezh State University, Voronezh, Russia., Pashkov AN; Department of Biology, Voronezh State Medical University named after N. N. Burdenko, Voronezh, Russia., Kuptsova GN; Department of Hospital Therapy and Endocrinology, Voronezh State Medical University Named After N.N. Burdenko, Voronezh, Russia.
Jazyk:	angličtina
Zdroj:	Minerva endocrinology [Minerva Endocrinol (Torino)] 2022 Sep; Vol. 47 (3), pp. 304-313. Date of Electronic Publication: 2022 Feb 23.
DOI:	10.23736/S2724-6507.20.03257-5
Abstrakt:	Background: Non-alcoholic fatty liver disease (NAFLD) is accompanied by inflammation and impairment of the lipid metabolism. In addition, NAFLD is one of the major complications of type 2 diabetes associated with oxidative stress. Based on this, we evaluated the tumor necrosis factor alpha (TNF-α), nuclear factor κB (NF-κB), oxidative status rates, and analyzed its correlation with carbohydrate and lipid metabolism in patients with NAFLD and type 2 diabetes. Methods: A case-control study included 63 participants with NAFLD developing in patients with type 2 diabetes, and 65 healthy volunteers with a normal complete blood count and blood biochemical profile. The following parameters and states were assessed during the study: glycaemia, insulin resistance, lipid levels, liver tests, intensity of free radical induced oxidation, antioxidant enzymes, TNF-α and NF-κB level. Results: Free radical induced oxidation was significantly elevated (P<0.001), total antioxidant activity was significantly decreased (P<0.001) and associated with insulin resistance (P=0.019) and lipid metabolism shifts (P<0.05) in patients with NAFLD and type 2 diabetes. Such patients had showed impaired functioning of antioxidant system (P<0.001), inhibition of NADPH-generating enzymes activity (P<0.001), increased levels of TNF-α (P<0.001) and NF-κB (P=0.019) correlated with the severity of hyperglycemia (P<0.05), concentration of reduced glutathione (P=0.005) and total cholesterol (P=0.016). Conclusions: The increase of free radical induced oxidation, TNF-α and NF-κB levels, and depletion of the antioxidant system seems to be the key factors of the development of NAFLD in patients with type 2 diabetes.
Databáze:	MEDLINE
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