| Autor: |
Farley SE, Kyle JE, Leier HC, Bramer LM, Weinstein J, Bates TA, Lee JY, Metz TO, Schultz C, Tafesse FG |
| Jazyk: |
angličtina |
| Zdroj: |
BioRxiv : the preprint server for biology [bioRxiv] 2022 Feb 15. Date of Electronic Publication: 2022 Feb 15. |
| DOI: |
10.1101/2022.02.14.480430 |
| Abstrakt: |
A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. We mapped alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We found that SARS-CoV-2 rewires host lipid metabolism, altering 409 lipid species up to 64-fold relative to controls. We correlated these changes with viral protein activity by transfecting human cells with each viral protein and performing lipidomics. We found that lipid droplet plasticity is a key feature of infection and that viral propagation can be blocked by small-molecule glycerolipid biosynthesis inhibitors. We found that this inhibition was effective against the main variants of concern (alpha, beta, gamma, and delta), indicating that glycerolipid biosynthesis is a conserved host dependency factor that supports this evolving virus. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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