Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy.

Autor: Neuberger EWI; Department of Sports Medicine, Rehabilitation and Disease Prevention, Faculty of Social Science, Media and Sport, Johannes Gutenberg-University Mainz, Albert-Schweitzerstr. 22, 55128, Mainz, Germany., Sontag S; Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Aachen, Germany.; Institute for Biomedical Engineering - Cell Biology, University Hospital of RWTH Aachen, Aachen, Germany., Brahmer A; Department of Sports Medicine, Rehabilitation and Disease Prevention, Faculty of Social Science, Media and Sport, Johannes Gutenberg-University Mainz, Albert-Schweitzerstr. 22, 55128, Mainz, Germany., Philippi KFA; Department of Sports Medicine, Rehabilitation and Disease Prevention, Faculty of Social Science, Media and Sport, Johannes Gutenberg-University Mainz, Albert-Schweitzerstr. 22, 55128, Mainz, Germany., Radsak MP; Department of Medicine III, Johannes Gutenberg University Medical Center, Mainz, Germany., Wagner W; Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Aachen, Germany.; Institute for Biomedical Engineering - Cell Biology, University Hospital of RWTH Aachen, Aachen, Germany., Simon P; Department of Sports Medicine, Rehabilitation and Disease Prevention, Faculty of Social Science, Media and Sport, Johannes Gutenberg-University Mainz, Albert-Schweitzerstr. 22, 55128, Mainz, Germany. simonpe@uni-mainz.de.
Jazyk: angličtina
Zdroj: Clinical epigenetics [Clin Epigenetics] 2022 Feb 22; Vol. 14 (1), pp. 29. Date of Electronic Publication: 2022 Feb 22.
DOI: 10.1186/s13148-022-01245-3
Abstrakt: Physical activity impacts immune homeostasis and leads to rapid and marked increase in cell-free DNA (cfDNA). However, the origin of cfDNA during exercise remains elusive and it is unknown if physical activity could improve or interfere with methylation based liquid biopsy. We analyzed the methylation levels of four validated CpGs representing cfDNA from granulocytes, lymphocytes, monocytes, and non-hematopoietic cells, in healthy individuals in response to exercise, and in patients with hematological malignancies under resting conditions. The analysis revealed that physical activity almost exclusively triggered DNA release from granulocytes, highlighting the relevance as a pre-analytical variable which could compromise diagnostic accuracy.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje