Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate.

Autor: Ku JT; Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 1001 Daxue Road, Hsinchu City, 300, Taiwan.; Institute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, 1001 Daxue Road, Hsinchu City, 300, Taiwan., Chen AY; Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, 1001 Daxue Road, Hsinchu City, 300, Taiwan.; Institute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, 1001 Daxue Road, Hsinchu City, 300, Taiwan., Lan EI; Department of Biological Science and Technology, National Chiao Tung University, 1001 Daxue Road, Hsinchu City, 300, Taiwan. ethanilan@nycu.edu.tw.; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, 1001 Daxue Road, Hsinchu City, 300, Taiwan. ethanilan@nycu.edu.tw.
Jazyk: angličtina
Zdroj: Microbial cell factories [Microb Cell Fact] 2022 Feb 22; Vol. 21 (1), pp. 28. Date of Electronic Publication: 2022 Feb 22.
DOI: 10.1186/s12934-022-01755-y
Abstrakt: Background: Butyl acetate is a versatile compound that is widely used in the chemical and food industry. The conventional butyl acetate synthesis via Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally benign. Alternative lipase-catalyzed ester formation requires a significant amount of organic solvent which also presents another environmental challenge. Therefore, a microbial cell factory capable of producing butyl acetate through fermentation of renewable resources would provide a greener approach to butyl acetate production.
Result: Here, we developed a metabolically engineered strain of Escherichia coli that efficiently converts glucose to butyl acetate. A modified Clostridium CoA-dependent butanol production pathway was used to synthesize butanol which was then condensed with acetyl-CoA through an alcohol acetyltransferase. Optimization of alcohol acetyltransferase expression and redox balance with auto-inducible fermentative controlled gene expression led to an effective titer of 22.8 ± 1.8 g/L butyl acetate produced in a bench-top bioreactor.
Conclusion: Building on the well-developed Clostridium CoA-dependent butanol biosynthetic pathway, expression of an alcohol acetyltransferase converts the butanol produced into butyl acetate. The results from this study provided a strain of E. coli capable of directly producing butyl acetate from renewable resources at ambient conditions.
(© 2022. The Author(s).)
Databáze: MEDLINE
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