Proteomic analysis of blood serum - a new approach to the search for diagnostic markers of bronchial asthma in children.

Autor:	Lebedenko AA; Rostov State Medical University., Afonin AA; Rostov State Medical University., Semernik OE; Rostov State Medical University., Loginova IG; Rostov State Medical University., Gunko VO; Rostov State Medical University., Larichkin AV; Rostov State Medical University., Alliluyev IA; Southern State University., Galkina GA; Rostov State Medical University., Panova IV; Rostov State Medical University.
Jazyk:	angličtina
Zdroj:	Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2022 Feb 23; Vol. 67 (2), pp. 81-84.
DOI:	10.51620/0869-2084-2022-67-2-81-84
Abstrakt:	Currently, bronchial asthma (BA) is one of the most pressing medical and social problems, the molecular aspects of the formation and development of BA are insufficiently studied and the diagnosis is not perfect. Carrying out proteomic analysis of BA will not only reveal new biomarkers specific to this disease, but also bring us closer to understanding its pathogenetic mechanisms. The purpose of the study: to study the proteomic profile of blood serum of children with BA to identify proteins associated with this disease A comprehensive clinical and laboratory examination of children suffering from BA and control group patients was performed. Proteomic analysis of depleted blood serum included high-resolution two-dimensional electrophoresis (1 direction: immobiline strips 17cm, pH 3-10, 2 direction: denaturing electrophoresis in 12.5% polyacrylamide gel), protein staining on gels with fluorescent dye Flamingo, protein identification by MALDI-TOF mass spectrometry using the search algorithm Mascot and the Swiss-Prot database. Comparison of the proteomic profile of BA serum and the control group patients serum allowed us to establish that the production of a number of proteins is reduced in this pathology. Among them, proteins in the molecular weight range of 16-33 kDa (p<0.05) were identified: glutathione peroxidase 3, transtyretin, complement components C4b and C3. Research shows that changes in the children's serum proteome occur in BA, affecting proteins that play an important role in immune responses, ligand transport, and antioxidant protection. Special attention should be paid to the differences identified in the course of this work (glutathione peroxidase, transtyretin, C3 and C4 fragments of the complement system) or their combinations. Studying the features of their expression will expand our understanding of the molecular mechanisms underlying chronic inflammation of this disease. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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