Antidiabetic activity of avocado seeds (Persea americana Mill.) in diabetic rats via activation of PI3K/AKT signaling pathway.

Autor: Ojo OA; Phytomedicine, Molecular Toxicology and Computational Biology Research Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria. oluwafemiadeleke08@gmail.com., Amanze JC; Phytomedicine, Molecular Toxicology and Computational Biology Research Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria., Oni AI; Phytomedicine, Molecular Toxicology and Computational Biology Research Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria., Grant S; Phytomedicine, Molecular Toxicology and Computational Biology Research Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria., Iyobhebhe M; Phytomedicine, Molecular Toxicology and Computational Biology Research Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria., Elebiyo TC; Phytomedicine, Molecular Toxicology and Computational Biology Research Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria., Rotimi D; Phytomedicine, Molecular Toxicology and Computational Biology Research Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria., Asogwa NT; Central Research Laboratory 123B, University Road, Tanke, Ilorin, Nigeria., Oyinloye BE; Department of Biochemistry, Afe Babalola University Ado-Ekiti, Ado-Ekiti, Nigeria., Ajiboye BO; Department of Biochemistry, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria., Ojo AB; Department of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Feb 21; Vol. 12 (1), pp. 2919. Date of Electronic Publication: 2022 Feb 21.
DOI: 10.1038/s41598-022-07015-8
Abstrakt: The treatment of diabetes involves the use of herbal plants, attracting interest in their cost-effectiveness and efficacy. An aqueous extract of Persea americana seeds (AEPAS) was explored in this study as a possible therapeutic agent in rats with diabetes mellitus. The induction of diabetes in the rats was achieved by injecting 65 mg/kg body weight (BWt) of alloxan along with 5% glucose. This study was conducted using thirty-six (36) male Wistar rats. The animals were divided into 6 equal groups, (n = 6) and treated for 14 days. In vitro assays for total flavonoid, phenols, FRAP, DPPH, NO, α-amylase, and α-glucosidase, were performed. Biochemical indices fasting blood sugar (FBS), BWt, serum insulin, liver hexokinase, G6P, FBP, liver glycogen, IL-6, TNF-α, and NF-ĸB in the serum, were investigated as well as the mRNA expressions of PCNA, Bcl2, PI3K/Akt in the liver and pancreas. The in vitro analyses showed the potency of AEPAS against free radicals and its enzyme inhibitory potential as compared with the positive controls. AEPAS showed a marked decrease in alloxan-induced increases in FBG, TG, LDL-c, G6P, F-1, 6-BP, MDA, IL-6, TNF-α, and NF-ĸB and increased alloxan-induced decreases in liver glycogen, hexokinase, and HDL-c. The diabetic control group exhibited pancreatic dysfunction as evidenced by a reduction in serum insulin, HOMA-β, expressions of PI3K/AKT, Bcl-2, and PCNA combined with an elevation in HOMA-IR. The HPLC revealed luteolin and myricetin to be the phytochemicals that were present in the highest concentration in AEPAS. The outcome of this research showed that the administration of AEPAS can promote the activation of the PI3K/AkT pathway and the inhibition of β-cell death, which may be the primary mechanism by which AEPAS promotes insulin sensitivity and regulates glycolipid metabolism.
(© 2022. The Author(s).)
Databáze: MEDLINE
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