Ocimum sanctum , Zingiber officinale, and Piper nigrum extracts and their effects on gut microbiota modulations (prebiotic potential), basal inflammatory markers and lipid levels: oral supplementation study in healthy rats.

Autor: Kondapalli NB; Department of Microbiology & Immunology, ICMR-National Institute of Nutrition, Hyderabad, Telangana State, India., Hemalatha R; Department of Microbiology & Immunology, ICMR-National Institute of Nutrition, Hyderabad, Telangana State, India., Uppala S; Dr. Pinnamaneni, Siddhartha Institute of Medical Sciences, Vijayawada, Andhra Pradesh, India., Yathapu SR; Department of Microbiology & Immunology, ICMR-National Institute of Nutrition, Hyderabad, Telangana State, India., Mohammed S; Department of Microbiology & Immunology, ICMR-National Institute of Nutrition, Hyderabad, Telangana State, India., Venkata Surekha M; Department of Pathology, ICMR-National Institute of Nutrition, Hyderabad, Telangana State, India., Rajendran A; Food Chemistry Division, ICMR-National Institute of Nutrition, Hyderabad, Telangana State, India., Bharadwaj DK; Food and Drug Toxicology department, ICMR-National Institute of Nutrition, Hyderabad, Telangana State, India.
Jazyk: angličtina
Zdroj: Pharmaceutical biology [Pharm Biol] 2022 Dec; Vol. 60 (1), pp. 437-450.
DOI: 10.1080/13880209.2022.2033797
Abstrakt: Context: Ocimum sanctum Linn (Labiatae) (OS), Zingiber officinale Rose (Zingiberaceae) (ZO), and Piper nigrum Linn (Piperaceae) (PN) are used in traditional medicine as immunomodulator, anti-inflammatory, and bioavailability enhancer agents.
Objective: Active phytoconstituents of OS, ZO, PN hydro-alcoholic extracts and their effects on gut microbiota, basal inflammation and lipid profile were investigated in rats.
Materials and Methods: Active phytoconstituents of extracts were analysed using HPLC and GC-MS. SD rats were supplemented with individual/combined extracts (OS-850; ZO-500; PN-100 mg/kg Bw) and Fructooligosaccharide (standard prebiotic-5g/kg-Bw), orally for 30 days. Haematology, lipid profile, LPS, CRP, IL-6, insulin and histology of vital organs were analysed. Caecal bacterial levels were assessed by RT-PCR.
Results: High content of phenolic compounds luteolin-7- O -glucoside (430 ± 2.3 mg/100g), gallic acid (84.13 ± 1.2 mg/100 g) and flavones (88.18 ± 1.8 mg/100 g) were found in OS, ZO, and PN, respectively. Combined extract was rich in luteolin-7- O -glucoside (266.0 ± 1.80 mg/100 g). Essential oils including methyleugenol (13.96%), 6-shogaol (11.00%), piperine (18.26%), and cyclopentasiloxane (10.06%) were higher in OS, ZO, PN and combined extract. Higher levels of caecal Lactobacillus (1.7-3.4-fold), Bifidobacterium (5.89-28.4-fold), and lower levels of Firmicutes (0.04-0.91-fold), Bacteroides (0.69-0.88-fold) were noted among extracts and FOS supplemented rats. Significant ( p <  0.05) decrease in plasma lipid profile and LPS was noted in all supplemented rats.
Discussion and Conclusions: The current study could be first of its kind in exploring prebiotic potential of OS, ZO, PN and their effect on native gut bacterial population.
Databáze: MEDLINE
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