Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice.

Autor: Winarni D; Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia., Husna FN; Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia., Syadzha MF; Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia., Susilo RJK; Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia., Hayaza S; Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia., Ansori ANM; Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.; Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia., Alamsjah MA; Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya 60115, Indonesia., Amin MNG; Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya 60115, Indonesia., Wulandari PAC; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia., Pudjiastuti P; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia., Awang K; Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
Jazyk: angličtina
Zdroj: Scientifica [Scientifica (Cairo)] 2022 Feb 09; Vol. 2022, pp. 9700794. Date of Electronic Publication: 2022 Feb 09 (Print Publication: 2022).
DOI: 10.1155/2022/9700794
Abstrakt: This research aimed to determine the topical administration effect of the combination of Sargassum duplicatum and Garcinia mangostana extracts to ameliorate diabetic open wound healing. The study used 24 adult males of Mus musculus (BALB/c strain, 3-4 months, 30-40 g). They were divided into normal control groups (KN) and diabetic groups. The diabetic group was streptozotocin-induced and divided further into three treatment groups: the diabetic control group (KD), the S. duplicatum treatment group (PA), and the combination of S. duplicatum and G. mangostana treatment group (PAM). The dose of treatment was 50 mg/kg of body weight. Each group was divided into three treatment durations, which were 3 days, 7 days, and 14 days. The wound healing process was determined by wound width, the number of neutrophils, macrophages, fibroblasts, fibrocytes, and collagen density. Histological observation showed that the topical administration of combination extracts increased the re-epithelialization of the wounded area, fibroblasts, fibrocytes, and collagen synthesis. The topical administration of combination extracts also decreased the number of neutrophils and macrophages. This study concluded that the topical administration of the combination of S. duplicatum and G. mangostana extracts improved the open wound healing process in diabetic mice.
Competing Interests: The authors declare that there are no conflicts of interest.
(Copyright © 2022 Dwi Winarni et al.)
Databáze: MEDLINE
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