Antibacterial and wound-healing potential of PLGA/spidroin nanoparticles: a study on earthworms as a human skin model.

Autor:	Abd El-Aziz FEA; Department of Zoology, Faculty of Science, Assiut University, Assiut, 71515, Egypt., Hetta HF; Department of Medical Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, 71526, Egypt., Abdelhamid BN; Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt., Abd Ellah NH; Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
Jazyk:	angličtina
Zdroj:	Nanomedicine (London, England) [Nanomedicine (Lond)] 2022 Mar; Vol. 17 (6), pp. 353-365. Date of Electronic Publication: 2022 Feb 21.
DOI:	10.2217/nnm-2021-0325
Abstrakt:	Aim: The essential protein element of spider silk 'spidroin' was used to assess its impact on the wound-healing process. Methods: Spidroin nanoparticles (NPs) were prepared using poly(lactic-co-glycolic acid) polymer (PLGA/spidroin NPs) at different weight ratios (5:1, 10:1, 15:1) and were in vitro characterized. The optimized NPs were tested in vitro for release and antibacterial activity. To assess wound-healing effects, NPs were topically applied on surgically induced injuries in Allolobophora caliginosa earthworms as a robust human skin model. Results: Optimized NPs (173 ± 3 nm) revealed considerable antibacterial effect against Staphylococcus aureus and Escherichia coli . After 4 days of NPs application on wounds, macroscopical and histological examinations revealed a significant reduction in wound and re-epithelialization times. Conclusion: PLGA/spidroin NPs may represent a promising option for wound repair.
Databáze:	MEDLINE
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