Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma.
Autor: | de Castro ACH; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil., Kochi LT; Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Flauzino JMR; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil., Soares MMCN; Adolfo Lutz Institute, Regional Laboratory in, São José Do Rio Preto, Brazil., Alves VA; Chemistry Department, Federal University of Triângulo Mineiro, Uberaba, Brazil., da Silva LA; Chemistry Department, Federal University of Triângulo Mineiro, Uberaba, Brazil., Madurro JM; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, Brazil., Brito-Madurro AG; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil. agbrito@ufu.br. |
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Jazyk: | angličtina |
Zdroj: | Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2022 Jun; Vol. 194 (6), pp. 2604-2619. Date of Electronic Publication: 2022 Feb 19. |
DOI: | 10.1007/s12010-022-03829-4 |
Abstrakt: | In this work, we report the construction of a novel electrochemical device for molecular diagnosis of hepatitis B virus in the blood plasma of infected patients, using graphite electrodes functionalized with poly(4-aminophenol) and sensitized with a specific DNA probe. The recognition of genomic DNA was evaluated by electrochemical techniques (DPV and EIS) and scanning electron microscopy. The genosensor was efficient in detecting genomic DNA with a linear range from 1.176 to 4.825 μg mL -1 and detection limit of 35.69 ng mL -1 (4.63 IU ml -1 or 25.93 copies.ml -1 ), which is better than the 10.00 IU ml -1 limit of reference method, real-time PCR, used in point of care. EIS analysis shows that the genosensor resistance increased exponentially with the concentration of the genomic DNA target. This novel platform has advantages to its applicability in real samples, such as good sensitivity, selectivity, low sample volume, and fast assay time (36 min), thus interesting for application in the diagnosis of hepatitis B virus in blood plasma. Also, the ease of synthesis of the low-cost polymer by electrosynthesis directly on the electrode surface allows the translation of the platform to portable devices. (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
Databáze: | MEDLINE |
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