RhoA, ROCK-1, and ROCK-2 Gene Expression and Polymorphisms in Cholesteatoma Patients.

Autor: Görür K; Department of Otorhinolaryngology Faculty of Medicine, University of Mersin, Mersin, Turkey., Büyükafşar K; Department of Pharmacology, Faculty of Medicine, University of Mersin, Mersin, Turkey., Akbaş E; Department of Medical Biology and Genetics Faculty of Medicine, University of Mersin, Mersin, Turkey., İsmi O; Department of Otorhinolaryngology Faculty of Medicine, University of Mersin, Mersin, Turkey., Yolal Ertural D; Department of Medical Biology and Genetics Faculty of Medicine, University of Mersin, Mersin, Turkey., Çetinkaya A; Department of Medical Biology and Genetics Faculty of Medicine, University of Mersin, Mersin, Turkey.
Jazyk: angličtina
Zdroj: The journal of international advanced otology [J Int Adv Otol] 2021 Nov; Vol. 17 (6), pp. 530-535.
DOI: 10.5152/iao.2021.21132
Abstrakt: Background: The aim of this study was to evaluate the gene polymorphism and expressions of Rho-A, ROCK-1, and ROCK-2 in cholesteatoma.
Methods: In this study, 120 healthy control group patients and 120 cholesteatoma patients were enrolled. Venous blood was taken from all of the cholesteatoma and control group patients. The genotyping of ROCK-1(G/T)rs35996865, ROCK-2(A/C)rs10178332, and Rho-A(A/T)rs2177268 polymorphisms was performed using predesigned TaqMan SNP Genotyping Assays. Assays-on-Demand SNP genotyping kit was used for the realtime polymerase chain reaction. The expression levels of Rho-A(Hs00357608_m1), ROCK-1(Hs01127699_m1), and ROCK-2(Hs00178154_m1) genes were determined.
Results: The expression of Rho-A, ROCK-1, and ROCK-2 was lower in cholesteatoma patients than in the control group. There was no difference in Rho-AAT/TT and ROCK-1GT/TT variation in cholesteatoma patients compared to the control. However, ROCK-2 AC/CC variance was lower in cholesteatoma patients.
Conclusion: The expression of Rho-A, ROCK-1, and ROCK-2 genes may be decreased in cholesteatoma. Furthermore, since ROCK-2 AC/CC genotype is also lower in cholesteatoma, having C allele seems to decrease the risk of developing this disease.
Databáze: MEDLINE
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