ABO antibody titres: a multisite comparative study of equivalency and reproducibility for automated solid-phase and haemagglutination titration, and manual dilution with gel column agglutination technology.

Autor:	Schneider D; Immucor Inc., Norcross, GA, United States of America., Vicarioto M; Department of Transfusion Medicine, University Hospital of Padua, Padua, Italy., Coluzzi S; Blood Transfusion Service and Haematology, Umberto I Hospital, Rome, Italy., Matteocci A; Transfusion Medicine Unit, San Camillo Forlanini Hospital, Rome, Italy., Revelli N; Department of Transfusion Medicine and Haematology and Lombardy Regional Rare Blood Bank, IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy., Foglieni B; Department of Transfusion Medicine and Haematology ASST Lecco, Lecco, Italy., Artusi P; Department of Transfusion Medicine, Azienda Ospedaliera Universitaria Policlinico, Modena, Italy., Londero D; Department of Transfusion Medicine, Azienda Sanitaria Universitaria Friuli Centrale, Udine, Italy., Quaglietta A; Department of Haematolgy and Oncology, Center of Transfusion Medicine, Pescara General Hospital, Pescara, Italy., Barrotta G; Department of Transfusion Medicine, Giovanni Paolo II Hospital, Ragusa, Italy., Visceglie D; Blood Transfusion Service, Di Venere Hospital Bari, Italy., Portararo G; Department of Transfusion Medicine, University Hospital, Parma, Italy., Gilsdorf J; Immucor Inc., Norcross, GA, United States of America.
Jazyk:	angličtina
Zdroj:	Blood transfusion = Trasfusione del sangue [Blood Transfus] 2022 Jul; Vol. 20 (4), pp. 329-337. Date of Electronic Publication: 2022 Jan 20.
DOI:	10.2450/2022.0197-21
Abstrakt:	Background: ABO antibody titres are important in many clinical decisions; however, much variability is observed in titre results. For reliable and reproducible titre results, automated ABO titration methods have been developed. In this 10-site study, we evaluated the equivalency of the automated ABO titration assays on the Galileo NEO, a fully automated blood bank analyzer (Immucor, Inc.) to manual titration with gel Column Agglutination Technology (CAT), as well as the reproducibility of both methods. Materials and Methods: Ten different locations participated in this study. The equivalency study included 70 random samples at each site. The reproducibility study tested the same blinded 30-sample panel at each study site. Anti-A and anti-B IgM and IgG antibody titres were tested with both the automated and manual methods; additionally, dithiothreitol (DTT) treatment was used to inactivate IgM antibodies in the manual CAT method. Results: The equivalency between CAT manual method and Galileo NEO automated titres at each site ranged from 38 to 88%; equivalency for each isotype was 66.2% for IgM, 60.6% for IgG, and 88.5% for DTT-treated IgG. The reproducibility study evaluated the titre variation of each sample obtained from the 10 sites. The average titre ranges (in doubling dilutions) for the automated and manual methods, respectively, were 2.15±1.0 and 4.03±1.8 for IgM, and 1.53±0.7 and 4.10±1.9 for IgG; for the manual DTT-treated IgG, the average titre range was 3.45±1.8 doubling dilutions. Discussion: The results demonstrated that the Galileo NEO automated and manual CAT ABO titres are not equivalent. However, the study also demonstrated that titre reproducibility is enhanced with the Galileo NEO automated ABO titration assays relative to the manual CAT ABO titration method. Therefore, to improve management of patients receiving care across multiple institutions, our study supports the use of automated ABO titration.
Databáze:	MEDLINE
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