Tomato CRABS CLAW paralogues interact with chromatin remodelling factors to mediate carpel development and floral determinacy.

Autor: Castañeda L; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, 04120, Spain., Giménez E; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, 04120, Spain., Pineda B; Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, 46022, Valencia, Spain., García-Sogo B; Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, 46022, Valencia, Spain., Ortiz-Atienza A; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, 04120, Spain., Micol-Ponce R; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, 04120, Spain., Angosto T; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, 04120, Spain., Capel J; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, 04120, Spain., Moreno V; Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, 46022, Valencia, Spain., Yuste-Lisbona FJ; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, 04120, Spain., Lozano R; Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, Almería, 04120, Spain.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2022 May; Vol. 234 (3), pp. 1059-1074. Date of Electronic Publication: 2022 Mar 03.
DOI: 10.1111/nph.18034
Abstrakt: CRABS CLAW (CRC) orthologues play a crucial role in floral meristem (FM) determinacy and gynoecium formation across angiosperms, the key developmental processes for ensuring successful plant reproduction and crop production. However, the mechanisms behind CRC mediated FM termination are far from fully understood. Here, we addressed the functional characterization of tomato (Solanum lycopersicum) paralogous CRC genes. Using mapping-by-sequencing, RNA interference and CRISPR/Cas9 techniques, expression analyses, protein-protein interaction assays and Arabidopsis complementation experiments, we examined their potential roles in FM determinacy and carpel formation. We revealed that the incomplete penetrance and variable expressivity of the indeterminate carpel-inside-carpel phenotype observed in fruit iterative growth (fig) mutant plants are due to the lack of function of the S. lycopersicum CRC homologue SlCRCa. Furthermore, a detailed functional analysis of tomato CRC paralogues, SlCRCa and SlCRCb, allowed us to propose that they operate as positive regulators of FM determinacy by acting in a compensatory and partially redundant manner to safeguard the proper formation of flowers and fruits. Our results uncover for the first time the physical interaction of putative CRC orthologues with members of the chromatin remodelling complex that epigenetically represses WUSCHEL expression through histone deacetylation to ensure the proper termination of floral stem cell activity.
(© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)
Databáze: MEDLINE
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