Isolation of CD35 + follicular dendritic cells and its role in the differentiation from B cells to IgA + GL7 + cells.

Autor: Hikosaka-Kuniishi M; Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan. Electronic address: hikos@med.mie-u.ac.jp., Yamane T; Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan., Isono K; Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan., Tetteh DN; Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan., Yamazaki H; Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
Jazyk: angličtina
Zdroj: Immunology letters [Immunol Lett] 2022 Mar; Vol. 243, pp. 53-60. Date of Electronic Publication: 2022 Feb 12.
DOI: 10.1016/j.imlet.2022.02.005
Abstrakt: Follicular dendritic cells (FDCs) are non-hematopoietic cells that are localized in the germinal centers (GCs) of lymph nodes (LNs) and are involved in humoral immunity. FDCs are a rare population that are sensitive to mechanical and chemical stimuli, making their isolation for analysis difficult. In Peyer's Patches, which are the main IgA-inductive sites, FDCs have been reported to be activated by retinoic acid receptor (RAR) and toll-like receptor (TLR) signals to induce IgA production. However, little is known about FDCs in mesenteric LNs (MLNs), although MLNs are also an IgA-inductive site. In this study, we efficiently isolated FDCs as CD35 + cells using anti-CD35 antibodies (Abs) and magnetic bead sorting. We found that CD35 +  FDCs facilitated differentiation from B220 + B cells into IgA + GL7 + GC B-like cells but not IgA + CD138 + plasma cells. Furthermore, using CD35 + FDCs from LPS-resistant C3H/HeJ mice, the generation of IgA + GL7 + GC B-like cells was not altered significantly between wild-type and LPS-resistant mice. Moreover, the addition of RAR antagonists and agonists revealed that differentiation into IgA + GL7 + GC B-like cells required the activation of RAR, especially RAR-β, in FDCs. The differentiation of IgA + GL7 + cells was promoted by FDCs in peripheral LNs as well as MLNs in our in vitro assay. Taken together, these results indicate that magnetic bead sorting with anti-CD35 Abs enable the efficient isolation of FDCs. Our data suggested that CD35 + FDCs can support differentiation of B cells into IgA + GL7 + GC B-like cells in environments that are not limited to MLNs, which can be stimulated by retinoic acid.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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