Blood Sampling for Arteriovenous Difference Measurements Across Interscapular Brown Adipose Tissue in Rat.

Autor:	Mestres-Arenas A; Departament de Bioquímica i Biomedicina Molecular, Universitat de Barcelona, Barcelona, Catalonia, Spain.; Institut de Recerca Sant Joan de Déu, Esplugues, Catalonia, Spain., Cairó M; Departament de Bioquímica i Biomedicina Molecular, Universitat de Barcelona, Barcelona, Catalonia, Spain.; Institut de Recerca Sant Joan de Déu, Esplugues, Catalonia, Spain.; CIBER Fisiopatología de la Obesidad y Nutrición, Madrid, Spain., Peyrou M; Departament de Bioquímica i Biomedicina Molecular, Universitat de Barcelona, Barcelona, Catalonia, Spain.; Institut de Recerca Sant Joan de Déu, Esplugues, Catalonia, Spain.; CIBER Fisiopatología de la Obesidad y Nutrición, Madrid, Spain., Villarroya F; Departament de Bioquímica i Biomedicina Molecular, Universitat de Barcelona, Barcelona, Catalonia, Spain. fvillarroya@ub.edu.; Institut de Recerca Sant Joan de Déu, Esplugues, Catalonia, Spain. fvillarroya@ub.edu.; CIBER Fisiopatología de la Obesidad y Nutrición, Madrid, Spain. fvillarroya@ub.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2448, pp. 273-282.
DOI:	10.1007/978-1-0716-2087-8_17
Abstrakt:	A classic physiological approach to assess the specific uptake or release of circulating factors in organs and tissues is to measure concentration differences between venous and arterial blood. For interscapular brown adipose tissue (iBAT), the anatomic distribution of its vascularization, which drains most of the blood into Sulzer's vein, allows for local measurement of arteriovenous differences. The use of this procedure to monitor oxygen concentration changes was fundamental for the recognition of BAT as the main site of adaptive non-shivering thermogenesis. More recently, this technique has regained importance as a means to identify BAT-secreted regulatory molecules, such as fibroblast growth factor-21 and the chemokine CXCL14. In this chapter, we provide a detailed description of an optimized and feasible protocol to determine arteriovenous differences across iBAT. We include tips and practical advice for using this powerful tool to study BAT metabolism and secretory activity in rats as an experimental model. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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