Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes.
| Autor: | Jung SM; Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA.; Department of Biological Sciences, Sungkyunkwan University, Suwon, South Korea., Le J; Department of Biological Chemistry, University of California Irvine, Irvine, CA, USA., Doxsey WG; Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA., Haley JA; Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA., Park G; Department of Biological Chemistry, University of California Irvine, Irvine, CA, USA., Guertin DA; Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA. David.Guertin@umassmed.edu., Jang C; Department of Biological Chemistry, University of California Irvine, Irvine, CA, USA. choljang@uci.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2448, pp. 119-130. |
| DOI: | 10.1007/978-1-0716-2087-8_8 |
| Abstrakt: | Brown adipose tissue (BAT) demonstrates extraordinary metabolic capacity. Previous research using conventional radio tracers reveals that BAT can act as a sink for a diverse menu of nutrients; still, the question of how BAT utilizes these nutrients remains unclear. Recent advances in mass spectrometry (MS) coupled to stable isotope tracing methods have greatly improved our understanding of metabolism in biology. Here, we have developed a BAT-tailored metabolomics and stable isotope tracing protocol using, as an example, the universally labeled 13 C-glucose, a key nutrient heavily utilized by BAT. This method enables metabolic roadmaps to be drawn and pathway fluxes to be inferred for each nutrient tracer within BAT and its application could uncover new metabolic pathways not previously appreciated for BAT physiology. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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