| Autor: |
Bolognesi F; Oral and Maxillofacial Surgery Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40136 Bologna, Italy., Fazio N; BST Biomedical Science and Technologies Lab, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy., Boriani F; Department of Plastic Surgery and Microsurgery, University of Cagliari, 09124 Cagliari, Italy., Fabbri VP; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40136 Bologna, Italy.; Unit of Anatomic Pathology, Department of Oncology, Bellaria 'Carlo Alberto Pizzardi' Hospital, Via Altura 3, 40139 Bologna, Italy., Gravina D; BST Biomedical Science and Technologies Lab, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy., Pedrini FA; Scuola di Specializzazione in Ortopedia e Traumatologia, Università degli Studi di Milano, Via Festa del Perdono 7, 20122 Milano, Italy.; IRCCS Istituto Ortopedico Galeazzi, Via Riccardo Galeazzi 4, 20161 Milano, Italy., Zini N; Unit of Bologna, CNR-National Research Council of Italy, Institute of Molecular Genetics 'Luigi Luca Cavalli-Sforza', Via di Barbiano 1/10, 40136 Bologna, Italy.; IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy., Greco M; BST Biomedical Science and Technologies Lab, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy., Paolucci M; Microbiology Section of the Department of Experimental, Diagnostic and Specialty Medicine, Azienda Ospedaliero-Universitaria di Bologna, Via Albertoni 15, 40138 Bologna, Italy., Re MC; Microbiology Section of the Department of Experimental, Diagnostic and Specialty Medicine, Azienda Ospedaliero-Universitaria di Bologna, Via Albertoni 15, 40138 Bologna, Italy., Asioli S; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40136 Bologna, Italy.; Unit of Anatomic Pathology, Department of Oncology, Bellaria 'Carlo Alberto Pizzardi' Hospital, Via Altura 3, 40139 Bologna, Italy., Foschini MP; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40136 Bologna, Italy.; Unit of Anatomic Pathology, Department of Oncology, Bellaria 'Carlo Alberto Pizzardi' Hospital, Via Altura 3, 40139 Bologna, Italy., D'Errico A; Pathology Unit, Department of Specialized, Experimental and Diagnostic Medicine, Azienda Ospedaliero-Universitaria di Bologna, Via Albertoni 15, 40138 Bologna, Italy., Baldini N; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40136 Bologna, Italy.; BST Biomedical Science and Technologies Lab, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy., Marchetti C; Oral and Maxillofacial Surgery Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40136 Bologna, Italy. |
| Abstrakt: |
Defects of the peripheral nervous system are extremely frequent in trauma and surgeries and have high socioeconomic costs. If the direct suture of a lesion is not possible, i.e., nerve gap > 2 cm, it is necessary to use grafts. While the gold standard is the autograft, it has disadvantages related to its harvesting, with an inevitable functional deficit and further morbidity. An alternative to autografting is represented by the acellular nerve allograft (ANA), which avoids disadvantages of autograft harvesting and fresh allograft rejection. In this research, the authors intend to transfer to human nerves a novel technique, previously implemented in animal models, to decellularize nerves. The new method is based on soaking the nerve tissues in decellularizing solutions while associating ultrasounds and freeze-thaw cycles. It is performed without interrupting the sterility chain, so that the new graft may not require post-production γ-ray irradiation, which is suspected to affect the structural and functional quality of tissues. The new method is rapid, safe, and inexpensive if compared with available commercial ANAs. Histology and immunohistochemistry have been adopted to evaluate the new decellularized nerves. The study shows that the new method can be applied to human nerve samples, obtaining similar, and, sometimes better, results compared with the chosen control method, the Hudson technique. |
