| Autor: |
Allakhverdiev ES; Russian National Medical Research Center of Cardiology, 3rd Cherepkovskaya St., 15A, 121552 Moscow, Russia.; Biology Faculty, Lomonosov Moscow State University, Leninskie Gory 1/12, 119991 Moscow, Russia., Khabatova VV; K.A. Timiryazev Institute of Plant Physiology, RAS, Botanicheskaya str., 35, 127276 Moscow, Russia., Kossalbayev BD; Geology and Oil-gas Business Institute Named after K. Turyssov, Satbayev University, Satpaeva, 22, Almaty 050043, Kazakhstan.; Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi Avenue 71, Almaty 050038, Kazakhstan., Zadneprovskaya EV; K.A. Timiryazev Institute of Plant Physiology, RAS, Botanicheskaya str., 35, 127276 Moscow, Russia., Rodnenkov OV; Russian National Medical Research Center of Cardiology, 3rd Cherepkovskaya St., 15A, 121552 Moscow, Russia., Martynyuk TV; Russian National Medical Research Center of Cardiology, 3rd Cherepkovskaya St., 15A, 121552 Moscow, Russia., Maksimov GV; Biology Faculty, Lomonosov Moscow State University, Leninskie Gory 1/12, 119991 Moscow, Russia.; Department of Physical Materials Science, Technological University 'MISiS', Leninskiy Prospekt 4, Office 626, 119049 Moscow, Russia., Alwasel S; Zoology Department, College of Science, King Saud University, Riyadh 12372, Saudi Arabia., Tomo T; Department of Biology, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan., Allakhverdiev SI; K.A. Timiryazev Institute of Plant Physiology, RAS, Botanicheskaya str., 35, 127276 Moscow, Russia.; Zoology Department, College of Science, King Saud University, Riyadh 12372, Saudi Arabia.; Institute of Basic Biological Problems, RAS, Pushchino, 142290 Moscow, Russia. |
| Abstrakt: |
Nowadays, there is an interest in biomedical and nanobiotechnological studies, such as studies on carotenoids as antioxidants and studies on molecular markers for cardiovascular, endocrine, and oncological diseases. Moreover, interest in industrial production of microalgal biomass for biofuels and bioproducts has stimulated studies on microalgal physiology and mechanisms of synthesis and accumulation of valuable biomolecules in algal cells. Biomolecules such as neutral lipids and carotenoids are being actively explored by the biotechnology community. Raman spectroscopy (RS) has become an important tool for researchers to understand biological processes at the cellular level in medicine and biotechnology. This review provides a brief analysis of existing studies on the application of RS for investigation of biological, medical, analytical, photosynthetic, and algal research, particularly to understand how the technique can be used for lipids, carotenoids, and cellular research. First, the review article shows the main applications of the modified Raman spectroscopy in medicine and biotechnology. Research works in the field of medicine and biotechnology are analysed in terms of showing the common connections of some studies as caretenoids and lipids. Second, this article summarises some of the recent advances in Raman microspectroscopy applications in areas related to microalgal detection. Strategies based on Raman spectroscopy provide potential for biochemical-composition analysis and imaging of living microalgal cells, in situ and in vivo. Finally, current approaches used in the papers presented show the advantages, perspectives, and other essential specifics of the method applied to plants and other species/objects. |
