Identification of Circulating lncRNAs Associated with Gallbladder Cancer Risk by Tissue-Based Preselection, Cis-eQTL Validation, and Analysis of Association with Genotype-Based Expression.

Autor: Blandino A; Statistical Genetics Research Group, Institute of Medical Biometry, Heidelberg University, 69120 Heidelberg, Germany., Scherer D; Statistical Genetics Research Group, Institute of Medical Biometry, Heidelberg University, 69120 Heidelberg, Germany., Rounge TB; Department of Research, Cancer Registry of Norway, 0379 Oslo, Norway.; Department of Informatics, University of Oslo, 0304 Oslo, Norway., Umu SU; Department of Research, Cancer Registry of Norway, 0379 Oslo, Norway., Boekstegers F; Statistical Genetics Research Group, Institute of Medical Biometry, Heidelberg University, 69120 Heidelberg, Germany., Barahona Ponce C; Statistical Genetics Research Group, Institute of Medical Biometry, Heidelberg University, 69120 Heidelberg, Germany., Marcelain K; Department of Basic and Clinical Oncology, Medical Faculty, University of Chile, Santiago 8380000, Chile., Gárate-Calderón V; Statistical Genetics Research Group, Institute of Medical Biometry, Heidelberg University, 69120 Heidelberg, Germany.; Department of Basic and Clinical Oncology, Medical Faculty, University of Chile, Santiago 8380000, Chile., Waldenberger M; Research Unit Molecular Epidemiology and Institute of Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany., Morales E; Hospital Regional de Talca, Talca 3460000, Chile.; Facultad de Medicina, Universidad Católica del Maule, Talca 3460000, Chile., Rojas A; Facultad de Medicina, Universidad Católica del Maule, Talca 3460000, Chile., Munoz C; Hospital Regional de Talca, Talca 3460000, Chile.; Facultad de Medicina, Universidad Católica del Maule, Talca 3460000, Chile., Retamales J; Instituto Nacional del Cáncer, Santiago 7500650, Chile., de Toro G; Hospital de Puerto Montt, Puerto Montt 5480000, Chile.; Escuela de Tecnología Médica, Universidad Austral de Chile sede Puerto Montt, Puerto Montt 5480000, Chile., Barajas O; Department of Basic and Clinical Oncology, Medical Faculty, University of Chile, Santiago 8380000, Chile.; Hospital Clínico Universidad de Chile, Santiago 8380456, Chile., Rivera MT; Hospital del Salvador, Santiago 7500922, Chile., Cortés A; Hospital del Salvador, Santiago 7500922, Chile., Loader D; Hospital Padre Hurtado, Santiago 8880456, Chile., Saavedra J; Hospital Padre Hurtado, Santiago 8880456, Chile., Gutiérrez L; Hospital San Juan de Dios, Santiago, 8320000, Chile., Ortega A; Hospital Regional, Arica 1000000, Chile., Bertrán ME; Unidad Registro hospitalario de Cáncer, Hospital Base Valdivia, Valdivia 5090146, Chile., Gabler F; Hospital San Borja Arriarán, Santiago 8320000, Chile., Campos M; Hospital San Borja Arriarán, Santiago 8320000, Chile., Alvarado J; Hospital Regional Guillermo Grant Benavente, Concepcion 4070386, Chile., Moisán F; Hospital Regional Guillermo Grant Benavente, Concepcion 4070386, Chile., Spencer L; Hospital Regional Guillermo Grant Benavente, Concepcion 4070386, Chile., Nervi B; Departamento de Hematología y Oncología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago 8330077, Chile., Carvajal-Hausdorf DE; Facultad de Medicina, Clínica Alemana Universidad del Desarrollo, Santiago 7650568, Chile., Losada H; Hospital de Temuco, Temuco 4780000, Chile., Almau M; Hospital de Rancagua, Rancagua 2820000, Chile., Fernández P; Hospital de Rancagua, Rancagua 2820000, Chile., Gallegos I; Department of Basic and Clinical Oncology, Medical Faculty, University of Chile, Santiago 8380000, Chile.; Hospital Clínico Universidad de Chile, Santiago 8380456, Chile., Olloquequi J; Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain.; Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Talca 3460000, Chile., Fuentes-Guajardo M; Departamento de Tecnología Médica, Facultad de Ciencias de la Salud, Tarapacá University, Arica 1000815, Chile., Gonzalez-Jose R; Instituto Patagónico de Ciencias Sociales y Humanas, Centro Nacional Patagónico, CONICET, Puerto Madryn U9120ACD, Argentina., Bortolini MC; Instituto de Biociências, Universidad Federal do Rio Grande do Sul, Puerto Alegre 15053, Brazil., Gallo C; Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 15102, Peru., Linares AR; Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Human Phenome Institute, Fudan University, Shanghai 200434, China.; ADES (Anthropologie Bio-Culturelle, Droit, Éthique et Santé), UFR de Médecine, Aix-Marseille University, 13007 Marseille, France.; Department of Genetics, Evolution and Environment, UCL Genetics Institute, University College London, London WC1E 6BT, UK., Rothhammer F; Instituto de Alta Investigación, Tarapacá University, Arica 1000000, Chile., Lorenzo Bermejo J; Statistical Genetics Research Group, Institute of Medical Biometry, Heidelberg University, 69120 Heidelberg, Germany.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2022 Jan 27; Vol. 14 (3). Date of Electronic Publication: 2022 Jan 27.
DOI: 10.3390/cancers14030634
Abstrakt: Long noncoding RNAs (lncRNAs) play key roles in cell processes and are good candidates for cancer risk prediction. Few studies have investigated the association between individual genotypes and lncRNA expression. Here we integrate three separate datasets with information on lncRNA expression only, both lncRNA expression and genotype, and genotype information only to identify circulating lncRNAs associated with the risk of gallbladder cancer ( GBC ) using robust linear and logistic regression techniques. In the first dataset, we preselect lncRNAs based on expression changes along the sequence "gallstones → dysplasia → GBC ". In the second dataset, we validate associations between genetic variants and serum expression levels of the preselected lncRNAs (cis-lncRNA-eQTLs) and build lncRNA expression prediction models. In the third dataset, we predict serum lncRNA expression based on individual genotypes and assess the association between genotype-based expression and GBC risk. AC084082.3 and LINC00662 showed increasing expression levels ( p -value = 0.009), while C22orf34 expression decreased in the sequence from gallstones to GBC ( p -value = 0.04). We identified and validated two cis-LINC00662-eQTLs (r 2 = 0.26) and three cis-C22orf34-eQTLs (r 2 = 0.24). Only LINC00662 showed a genotyped-based serum expression associated with GBC risk (OR = 1.25 per log2 expression unit, 95% CI 1.04-1.52, p -value = 0.02). Our results suggest that preselection of lncRNAs based on tissue samples and exploitation of cis-lncRNA-eQTLs may facilitate the identification of circulating noncoding RNAs linked to cancer risk.
Databáze: MEDLINE
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