Matrix metalloproteinases and their inhibitors in pediatric severe acute pancreatitis.

Autor: Vitale DS; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio.; Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio., Lahni P; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio.; Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio., Hornung L; Division of Biostatistics and Epidemiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio., Thompson T; Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio., Farrell PR; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio.; Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio., Lin TK; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio.; Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio., Nathan JD; Division of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.; Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio., Wong HR; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio.; Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio., Abu-El-Haija M; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio.; Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Feb 14; Vol. 17 (2), pp. e0261708. Date of Electronic Publication: 2022 Feb 14 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0261708
Abstrakt: Background: Acute pancreatitis (AP) is increasing in incidence in adult and pediatric patients. Identification of patients at high risk for progression to severe acute pancreatitis (SAP) is crucial, as it can lead to increased mortality and health system cost. Matrix metalloproteinases (MMPs) are endopeptidases which degrade extracellular matrix proteins and increase activity of pro-inflammatory cytokines. Tissue inhibitors of metalloproteinases (TIMPs) regulate MMP activity. Prior limited studies of MMPs and TIMPs have found some to be associated with development of SAP. The aim of this study was to further investigate the role of MMPs and TIMPs in detecting pediatric patients at risk for developing moderately severe AP or SAP.
Methods: Plasma samples were prospectively collected for patients <21 years of age presenting with AP between November 2015 and October 2019, along with healthy controls. Bead-based multiplex assays were utilized to test levels of 12 MMPs and TIMPs.
Results: Samples were collected from 7 subjects who developed SAP, 7 with moderately severe AP, 45 with mild AP and 44 healthy controls. MMP-9 (p = 0.04) and TIMP-1 (p = 0.01) levels were significantly higher in SAP patients. A multivariable logistic regression model using MMP-9 and TIMP-1 predicted SAP (AUROC 0.87, 95% CI 0.76-0.98).
Conclusion: We have demonstrated that MMP9 and TIMP1 levels are increased at AP presentation in pediatric patients who developed SAP during the course of illness. Further studies are needed to validate the use of MMPs and TIMPs as predictive tools for development of SAP in pediatric pancreatitis.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje