Characterization and biological activities of melanin pigment from root endophytic fungus, Phoma sp. RDSE17.

Autor: Surendirakumar K; Department of Biotechnology, JJ College of Arts and Science (Autonomous), Tamil Nadu, Pudukkottai, 622 422, India. surenderpbt@gmail.com.; Department of Life Sciences, Manipur University, Canchipur, Imphal, 795 003, Manipur, India. surenderpbt@gmail.com., Pandey RR; Department of Life Sciences, Manipur University, Canchipur, Imphal, 795 003, Manipur, India., Muthukumar T; Department of Botany, Bharathiar University, Tamil Nadu, Coimbatore, 641 046, India., Sathiyaseelan A; Centre for Advanced Studies in Botany, University of Madras, Tamil Nadu, Chennai, 600 025, India., Loushambam S; Department of Life Sciences, Manipur University, Canchipur, Imphal, 795 003, Manipur, India., Seth A; Department of Life Sciences, Manipur University, Canchipur, Imphal, 795 003, Manipur, India.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2022 Feb 14; Vol. 204 (3), pp. 171. Date of Electronic Publication: 2022 Feb 14.
DOI: 10.1007/s00203-022-02788-y
Abstrakt: Melanins are high molecular weight hydrophobic pigments which have gained popularity for their role in virulence against different pathogens. In the present study, we isolated and characterized the melanin pigment produced by a dark septate endophyte fungus Phoma sp. RDSE17, which was associated with the roots of an indigenous Oryza sativa cv. 'Chakhao amubi' in Manipur, Northeast India. The biological properties of purified melanin from the fungus were evaluated for their antioxidant, antimicrobial and anticancerous activities. The pigment was extracted from Phoma sp. by alkaline-acid hydrolysis method and confirmed as melanin through physico-chemical tests and spectral (UV, FTIR, and EPR) analysis. The analyses of the elemental composition indicated that the pigment possessed a low percentage of nitrogen (N) contents, and therefore, would not fall under DOPA class of melanin. Exposure of the fungus to melanin pathway inhibitors revealed a positive melanin inhibition by tricyclazole, but not by kojic acid. Thus, the melanin from Phoma sp. may be a member of the DHN family. Moreover, the purified melanin showed high DPPH (1, 1-Diphenyl-2-picrylhydrazyl) free radical-scavenging activity with an EC 50 of 69 µg/mL and inhibited human lung cancer cell (A549 cells) proliferation at 80 µg/mL. The present study demonstrates that melanin from Phoma sp. RDSE17 could be employed as a potential biological (antioxidant) and antimicrobial agent for inhibiting the growth of humans and phytopathogens.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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