Antibacterial Effects of a 940 nm Diode Laser With/ Without Silver Nanoparticles Against Enterococcus faecalis .

Autor:	Hendi SS; Department of Endodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran., Shiri M; General Dentist, Hamadan University of Medical Sciences, Hamadan, Iran., Poormoradi B; Dental Research Center, Department of Periodontology, Dentistry Faculty, Hamadan University of Medical Sciences, Hamadan, Iran., Alikhani MY; Faculty of Medicine, Microbiology Department, Hamadan University of Medical Sciences, Hamadan, Iran., Afshar S; Department of Molecular Medicine and Genetics, School of Advanced Technologies in Medicine, Molecular Medicine Research Center, Hamadan University of Medical Sciences, Hamadan, Iran., Farmani A; Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Jazyk:	angličtina
Zdroj:	Journal of lasers in medical sciences [J Lasers Med Sci] 2021 Nov 24; Vol. 12, pp. e73. Date of Electronic Publication: 2021 Nov 24 (Print Publication: 2021).
DOI:	10.34172/jlms.2021.73
Abstrakt:	Introduction: The final goal of root canal therapy is to remove of the most bacteria from the root canal. This study aimed at comparing the antibacterial effects of a diode laser with a wavelength of 940nm and silver nanoparticles and the synergic effects of both techniques on Enterococcus faecalis . Methods: Ninety single-rooted human teeth were decoronated and prepared with rotary files. The samples were irrigated with sodium hypochlorite and EDTA17%. Then they were autoclaved and contaminated with E. faecalis suspension (1.5 × 10 8 CFU/mL) for 21 days. The samples were fixed in a microtube and were randomly divided into 4 experimental (n=20) groups and a negative control group (n=10) as follows: Group 1: hypochlorite sodium 5%, Group 2: silver nanoparticle, Group 3: diode laser, and Group 4: diode laser and silver nanoparticle. The samples were obtained from dentin chips before and after the intervention. The data were analysed using the Kruskal-Wallis nonparametric test. Furthermore, alterations in bacterial colonies were entered using the Wilcoxon signed ranks test (α=0.05). Results: There was a significant decrease in colony counts for all groups after interventions ( P value<0.05). Also, all groups showed more reductions in colony counts compared with the negative control group ( P value <0.004). There was a significant reduction for group 1 in comparison with other groups ( P value <0.001) and this group had an extreme decrease of colony counts (RCC=100%). There was an important differential between silver nanoparticles and diode laser groups in bacterial counts ( P value<0.001) and silver nanoparticles (RCC=83.15%) had more efficiency than the diode laser (RCC=41/33%). RCC of group 4 was 68/52%. Conclusion: Followed by sodium hypochlorite 5%, silver nanoparticles were the most effective antibacterial substances. The 940 nm laser diode had less antibacterial effect compared to its use with silver nanoparticles. (Copyright © 2021 J Lasers Med Sci.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu