Exosomal miRNA-328-3p targets ZO-3 and inhibits porcine epidemic diarrhea virus proliferation.

Autor: Zhao H; College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China., Yang J; Jilin Provincal Center for Animal Disease Control and Prevention, Changchun, 130117, Jilin, China., Wang Q; Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, 130117, Jilin, China., Cui Z; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, Gansu, China., Li D; College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang, 712100, Shanxi, China., Niu J; College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China., Guo Y; College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China., Zhang Q; College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China., Zhang S; College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China., Zhao Y; College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China., Wang K; College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China. wk197811@126.com., Lian W; Jilin ZhengYe Biological Products Co., Ltd., Jilin, 132101, Jilin, China. 5196602@qq.com., Hu G; College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China. huguixue901103@163.com.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2022 Mar; Vol. 167 (3), pp. 901-910. Date of Electronic Publication: 2022 Feb 11.
DOI: 10.1007/s00705-022-05364-3
Abstrakt: As essential transfer carriers for cell-to-cell communication and genetic material, exosomes carry microRNAs that participate in the regulation of various biological processes. MicroRNAs are a type of single-stranded noncoding RNA that bind to specific target gene mRNAs to degrade or inhibit their translation, thereby regulating target gene expression. Although it is known that a variety of microRNAs are involved in the viral infection process, there are few reports on specific microRNAs involved in porcine epidemic diarrhea virus (PEDV) infection. In this study, we isolated and identified exosomes in PEDV-infected Vero E6 cells. Using transcriptomics technology, we found that miRNA-328-3p was significantly downregulated in exosomes following PEDV infection. Moreover, exosomal miRNA-328-3p inhibited infection by PEDV by targeting and inhibiting tight junction protein 3 (TJP-3/ZO-3) in recipient cells. Our findings provide evidence that, after infecting cells, PEDV downregulates expression of miRNA-328-3p, and the resulting reduced inhibition of the target protein ZO-3 helps to enhance PEDV infection. These results provide new insight for understanding the regulatory mechanism of PEDV infection.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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