Detection of galanin receptors in the spinal cord in experimental autoimmune encephalomyelitis.

Autor:	Michalickova D; Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic., Kramarikova I; Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic., Ozturk HK; Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic., Kucera T; Institute of Histology and Embryology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic., Vacik T; Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic., Hrncir T; Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czech Republic., Kutinova Canova N; Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic., Sima M; Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic., Slanar O; Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic.
Jazyk:	angličtina
Zdroj:	Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia [Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub] 2023 Mar; Vol. 167 (1), pp. 36-42. Date of Electronic Publication: 2022 Feb 11.
DOI:	10.5507/bp.2022.005
Abstrakt:	Aims: The neuropeptide galanin is a widely distributed neurotransmitter/neuromodulator that regulates a variety of physiological processes and also participates in the regulation of stress responses. The aims of the present study were to investigate the expression of galanin receptors (GalR1, GalR2, GalR3) in the spinal cords in a murine model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE) using qPCR analysis and to determine GalR1 cellular localization (oligodendrocytes, microglia, astrocytes, ependymal cells, and endothelial cells in the capillaries) by immunohistochemistry. Methods: Twelve samples from the EAE group and 14 samples from the control group were analyzed. Spinal cords samples were obtained at the peak of the EAE disease. Results: The GalR1 mRNA level was significantly decreased in the EAE mice compared with the controls (P=0.016), whereas the mRNA levels of GalR2 and GalR3 were not significantly different for the EAE and the control mice. No significant correlations were found between the severity of the EAE disease and the mRNA levels of GalR1, GalR2 and GalR3. Immunochemical detection of the GalR1 revealed its expression in the ependymal and endothelial cells. Additionally, a weak GalR1 immunoreactivity was occasionally detected in the oligodendrocytes. Conclusion: This study provides additional evidence of galanin involvement in EAE pathophysiology, but this has to be further investigated. Competing Interests: The authors report no conflicts of interest in this work.
Databáze:	MEDLINE
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