A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma.
| Autor: | Machado ES; Pain Medical Center, Regenerar, Porto Alegre, BRA.; Faculty of Medicine, University of Porto, Porto, PRT., Soares FP; Pain Medical Center, Regenerar, Porto Alegre, BRA., Yamaguchi RS; Departamento de Ciências Fisiológicas, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, BRA., Felipone WK; Departamento de Ciências Fisiológicas, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, BRA., Meves R; Santa Casa Spine Center, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, BRA., Souza TAC; Pain Medical Center, Regenerar, Porto Alegre, BRA., Topolniak R; Santa Casa Spine Center, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, BRA., Caldas JP; Public Health Institute, University of Porto, Porto, PRT., Abreu EV; Pain Medical Center, Regenerar, Porto Alegre, BRA., Rabelo Neto LS; Spine Group, Hospital Ernesto Dornelles, Porto Alegre, BRA., Pinchemel PVS; Spine Group, Hospital Ernesto Dornelles, Porto Alegre, BRA., Bredemeier M; Serviço de Reumatologia, Hospital Nossa Senhora da Conceição, Porto Alegre, BRA. |
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| Jazyk: | angličtina |
| Zdroj: | Cureus [Cureus] 2022 Jan 03; Vol. 14 (1), pp. e20899. Date of Electronic Publication: 2022 Jan 03 (Print Publication: 2022). |
| DOI: | 10.7759/cureus.20899 |
| Abstrakt: | Objective: To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature. Methods: Sixteen blood samples from healthy volunteers were collected. PRP was prepared using our new double-spin technique, consisting of successive centrifugation of blood samples with two different spins, without opening the container. Descriptive analysis of cell counts in baseline and PRP samples was undertaken. Comparison between cell and platelet count in baseline and PRP samples, as well as the statistical analysis, were done. Results: The mean platelet concentration ratio was 3.47 (SD: 0.85; 95% CI: 3.01-3.92; range: 2.48-5.71). The baseline whole blood platelet count correlated positively to the PRP platelet count (rP Conclusion: Results show a safe and easily reproducible method to obtain PRP for use in clinical daily practice. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2022, Machado et al.) |
| Databáze: | MEDLINE |
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