In Situ Enhancement and Isotopic Labeling of Biogenic Coalbed Methane.

Autor: Barnhart EP; U.S. Geological Survey, Helena, Montana 59601, United States.; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States., Ruppert LF; U.S. Geological Survey, Reston, Virginia 20192, United States., Hiebert R; Biosqueeze Inc., Butte, Montana 59701, United States., Smith HJ; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States.; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, United States., Schweitzer HD; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States.; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, United States., Clark AC; U.S. Geological Survey, Reston, Virginia 20192, United States., Weeks EP; U.S. Geological Survey, Reston, Virginia 20192, United States., Orem WH; U.S. Geological Survey, Reston, Virginia 20192, United States., Varonka MS; U.S. Geological Survey, Reston, Virginia 20192, United States., Platt G; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States.; Department of Chemical and Biological Engineering, Montana State University, Bozeman, Montana 59717, United States., Shelton JL; U.S. Geological Survey, Reston, Virginia 20192, United States., Davis KJ; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States.; Department of Chemical and Biological Engineering, Montana State University, Bozeman, Montana 59717, United States., Hyatt RJ; Biosqueeze Inc., Butte, Montana 59701, United States., McIntosh JC; Department of Hydrology and Atmospheric Sciences, University of Arizona, Tucson, Arizona 85721, United States., Ashley K; Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States., Ono S; Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States., Martini AM; Geology Department, Amherst College, Amherst, Massachusetts 01002, United States., Hackley KC; Isotech/Stratum Reservoir, Champaign, Illinois 61821, United States., Gerlach R; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States.; Department of Chemical and Biological Engineering, Montana State University, Bozeman, Montana 59717, United States.; Isotech/Stratum Reservoir, Champaign, Illinois 61821, United States., Spangler L; Energy Research Institute, Montana State University, Bozeman, Montana 59717, United States., Phillips AJ; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States.; Department of Chemical and Biological Engineering, Montana State University, Bozeman, Montana 59717, United States.; Isotech/Stratum Reservoir, Champaign, Illinois 61821, United States., Barry M; Pro-Oceanus Systems Inc., Bridgewater, Nova Scotia B4V 1N1, Canada., Cunningham AB; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States., Fields MW; Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United States.; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, United States.
Jazyk: angličtina
Zdroj: Environmental science & technology [Environ Sci Technol] 2022 Mar 01; Vol. 56 (5), pp. 3225-3233. Date of Electronic Publication: 2022 Feb 10.
DOI: 10.1021/acs.est.1c05979
Abstrakt: Subsurface microbial (biogenic) methane production is an important part of the global carbon cycle that has resulted in natural gas accumulations in many coal beds worldwide. Laboratory studies suggest that complex carbon-containing nutrients (e.g., yeast or algae extract) can stimulate methane production, yet the effectiveness of these nutrients within coal beds is unknown. Here, we use downhole monitoring methods in combination with deuterated water (D 2 O) and a 200-liter injection of 0.1% yeast extract (YE) to stimulate and isotopically label newly generated methane. A total dissolved gas pressure sensor enabled real-time gas measurements (641 days preinjection and for 478 days postinjection). Downhole samples, collected with subsurface environmental samplers, indicate that methane increased 132% above preinjection levels based on isotopic labeling from D 2 O, 108% based on pressure readings, and 183% based on methane measurements 266 days postinjection. Demonstrating that YE enhances biogenic coalbed methane production in situ using multiple novel measurement methods has immediate implications for other field-scale biogenic methane investigations, including in situ methods to detect and track microbial activities related to the methanogenic turnover of recalcitrant carbon in the subsurface.
Databáze: MEDLINE
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