Ultrasensitive detection of plant hormone abscisic acid-based surface-enhanced Raman spectroscopy aptamer sensor.

Autor: Zhang Y; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China.; Henan International Joint Laboratory of Laser Technology in Agricultural Sciences, Zhengzhou, 450002, China., Li L; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China.; Henan International Joint Laboratory of Laser Technology in Agricultural Sciences, Zhengzhou, 450002, China., Zhang H; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China.; Henan International Joint Laboratory of Laser Technology in Agricultural Sciences, Zhengzhou, 450002, China., Shang J; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China.; Henan International Joint Laboratory of Laser Technology in Agricultural Sciences, Zhengzhou, 450002, China., Li C; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China.; Henan International Joint Laboratory of Laser Technology in Agricultural Sciences, Zhengzhou, 450002, China., Naqvi SMZA; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China.; Henan International Joint Laboratory of Laser Technology in Agricultural Sciences, Zhengzhou, 450002, China., Birech Z; Department of Physics, University of Nairobi, Nairobi, 30197, Kenya., Hu J; College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China. jdhu@henau.edu.cn.; Henan International Joint Laboratory of Laser Technology in Agricultural Sciences, Zhengzhou, 450002, China. jdhu@henau.edu.cn.; State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 45002, China. jdhu@henau.edu.cn.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2022 Mar; Vol. 414 (8), pp. 2757-2766. Date of Electronic Publication: 2022 Feb 10.
DOI: 10.1007/s00216-022-03923-w
Abstrakt: Abscisic acid (ABA), as the most common plant hormone in the growth of wheat, can greatly affect the yield when its levels deviate from normal. Therefore, highly sensitive and selective detection of this hormone is greatly needed. In this work, we developed an aptamer sensor based on surface-enhanced Raman spectroscopy (SERS) and applied it for the high sensitivity detection of ABA. Biotin-modified ABA aptamer complement chains were modified on ferrosoferric oxide magnetic nanoparticles (Fe 3 O 4 MNPs) and acted as capture probes, and sulfhydryl aptamer (SH-Apt)-modified silver-coated gold nanospheres (Au@Ag NPs) were used as signal probes. Through the recognition of the ABA aptamer and its complementary chains, an aptamer sensor based on SERS was constructed. As SERS internal standard molecules of 4-mercaptobenzoic acid (4-MBA) were encapsulated between the gold core and silver shell of the signal probes; the constructed aptamer sensor generated a strong SERS signal of 4-MBA after magnetic separation. When there were ABA molecules in the detection system, with the preferential binding of ABA aptamer and ABA molecule, the signal probes were released from the capture probes, after magnetic separation, leading to a linear decrease in SERS intensity of 4-MBA. Thus, the detection response was linear over a logarithmic concentration range, with an ultra-low detection limit of 0.67 fM. In addition, the practical use of this assay method was demonstrated in ABA detection from fresh wheat leaves, with a relative error (RE) of 5.43-8.94% when compared with results from enzyme-linked immunosorbent assay (ELISA). The low RE value proves that the aptamer sensor will be a promising method for ABA detection.
(© 2022. Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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