Determination of zilpaterol in a residue depletion study using LC-MS/MS in cattle plasma, muscle, liver and kidney.

Autor: Dolores-Hernández M; Laboratory of Pharmaceutical Development Tests, Multidisciplinary Research Unit (UIM), Faculty of Higher Education Cuautitlán, National Autonomous University of Mexico, Carr. Cuautitlán-Teoloyucan Km 2.5, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Mexico., Morales-Hipólito EA; Laboratory of Pharmaceutical Development Tests, Multidisciplinary Research Unit (UIM), Faculty of Higher Education Cuautitlán, National Autonomous University of Mexico, Carr. Cuautitlán-Teoloyucan Km 2.5, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Mexico., Villaseñor A; IMMA, Instituto de Medicina Molecular Aplicada, Facultad de Medicina, San Pablo CEU Universities, Madrid, España., López-Arellano R; Laboratory of Pharmaceutical Development Tests, Multidisciplinary Research Unit (UIM), Faculty of Higher Education Cuautitlán, National Autonomous University of Mexico, Carr. Cuautitlán-Teoloyucan Km 2.5, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Mexico. Electronic address: lopezar@unam.mx.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2022 Jul 15; Vol. 382, pp. 132287. Date of Electronic Publication: 2022 Jan 31.
DOI: 10.1016/j.foodchem.2022.132287
Abstrakt: Zilpaterol is a β-agonist compound which promotes fat loss and muscle gain in cattle, providing economic benefits. However, zilpaterol residues in the animal might introduce a significant risk to humans after consumption. In the present manuscript, a highly specific, sensitive method using Selected Reaction Monitoring (SRM) in positive electrospray ionization (ESI +) mode by liquid chromatography coupled to triple quadrupole mass spectrometry (LC-MS/MS) for plasma, muscle, liver and kidney is presented. For method development, composition of the aqueous mobile phase, precipitation agent, and solid phase extraction (SPE) conditions were optimized. The method was fully validated showing a good linearity and recovery average greater than or equal to 97 % for all matrices. The method was applied to residue depletion studies in cattle after withdrawal of zilpaterol supplementation at 3, 4, 5 and 6 days showing that tissues can be consumed by humans after 4th day of zilpaterol withdrawal.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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