| Autor: |
He Y; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States., Brademan DR; Morgridge Institute for Research, Madison, Wisconsin 53715, United States., Hutchins PD; Morgridge Institute for Research, Madison, Wisconsin 53715, United States., Overmyer KA; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.; Morgridge Institute for Research, Madison, Wisconsin 53715, United States., Coon JJ; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.; Morgridge Institute for Research, Madison, Wisconsin 53715, United States.; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States. |
| Abstrakt: |
Liquid chromatography-mass spectrometry (LC-MS) is a typical strategy for lipidomics analysis. Although capillary LC-MS is a common analytical technique for proteomics analysis, its application to lipidomics has been limited. In this study, we aim at improving lipid identifications achieved in a single LC-MS analysis by a 3-fold approach: capillary LC and nanoelectrospray for enhanced ionization, ion trap for higher sensitivity tandem MS, and parallelization of mass analyzers for increased speed of acquisition on an Orbitrap hybrid system. By applying the methods to a complex lipid mixture of human plasma, we identified and performed relative quantification on over 1500 lipids within a 60 min capillary LC-MS analysis. |
