| Autor: |
Vera C; Instituto de Bionanotecnolgía del NOA (INBIONATEC), CONICET, Universidad Nacional de Santiago del Estero (UNSE), RN9, km 1125, Santiago del Estero G4206XCP, Argentina., Gallucci MN; Instituto de Bionanotecnolgía del NOA (INBIONATEC), CONICET, Universidad Nacional de Santiago del Estero (UNSE), RN9, km 1125, Santiago del Estero G4206XCP, Argentina., Marioni J; CONICET, Instituto Multidisciplinario de Biología Vegetal (IMBIV), Cordoba X5000HUA, Argentina., Sosa Morales MC; Instituto de Bionanotecnolgía del NOA (INBIONATEC), CONICET, Universidad Nacional de Santiago del Estero (UNSE), RN9, km 1125, Santiago del Estero G4206XCP, Argentina., Martino DM; Instituto de Física del Litoral (IFIS Litoral), CONICET, Universidad Nacional del Litoral (UNL), Santa Fe S3000GLN, Argentina., Nuñez Montoya S; CONICET, Instituto Multidisciplinario de Biología Vegetal (IMBIV), Cordoba X5000HUA, Argentina.; Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Dpto. Cs. Farmacéuticas, Haya de la Torre y Medina Allende, Córdoba X5000HUA, Argentina., Borsarelli CD; Instituto de Bionanotecnolgía del NOA (INBIONATEC), CONICET, Universidad Nacional de Santiago del Estero (UNSE), RN9, km 1125, Santiago del Estero G4206XCP, Argentina.; Facultad de Agronomía y Agroindustrias. UNSE, Av. Belgrano (S) 1912, Santiago del Estero G4200, Argentina. |
| Abstrakt: |
The antimicrobial photodynamic activity (aPDA) in fungal and bacterial strains of supramolecular adducts formed between the anionic photosensitizer (PS) Rose Bengal (RB 2- ) and aromatic polycations derived from ( p -vinylbenzyl)triethylammonium chloride was evaluated. Stable supramolecular adducts with dissociation constants K d ≈ 5 μM showed photosensitizing properties suitable for generating singlet oxygen (Φ Δ = 0.5 ± 0.1) with the added advantage of improving the photostability of the xanthenic dye. However, the aPDA of both free and supramolecular RB 2- was highly dependent on the type of microorganism treated, indicating the importance of specific interactions between the different cell wall structures of the microbe and the PSs. Indeed, in the case of Gram-positive Staphylococcus aureus , the aPDA of molecular and supramolecular PSs was highly effective. Instead, in the case of Gram-negative Escherichia coli, only the RB 2- :polycation adducts showed aPDA, while RB 2- alone was inefficient, but in the case of Candida tropicalis, the opposite behavior was observed. Therefore, the present results indicate the potential of supramolecular chemistry to obtain aPDA à la carte depending on the target microbe and the PS properties. |
