A chromosomal-level reference genome of the widely utilized Coccidioides posadasii laboratory strain "Silveira".

Autor: de Melo Teixeira M; Faculty of Medicine, University of Brasília, Brasília 70910-900, Brazil.; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA., Stajich JE; Institute for Integrative Genome Biology, University of California Riverside, Riverside, CA 92521, USA.; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA 92521, USA., Sahl JW; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA., Thompson GR; Department of Medical Microbiology and Immunology, University of California Davis, Davis, CA 95616, USA., Brem RB; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, USA., Dubin CA; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, USA., Blackmon AV; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA., Mead HL; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA., Keim P; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA., Barker BM; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2022 Apr 04; Vol. 12 (4).
DOI: 10.1093/g3journal/jkac031
Abstrakt: Coccidioidomycosis is a common fungal disease that is endemic to arid and semi-arid regions of both American continents. Coccidioides immitis and Coccidioides posadasii are the etiological agents of the disease, also known as Valley Fever. For several decades, the C. posadasii strain Silveira has been used widely in vaccine studies, is the source strain for production of diagnostic antigens, and is a widely used experimental strain for functional studies. In 2009, the genome was sequenced using Sanger sequencing technology, and a draft assembly and annotation were made available. In this study, the genome of the Silveira strain was sequenced using single molecule real-time sequencing PacBio technology, assembled into chromosomal-level contigs, genotyped, and the genome was reannotated using sophisticated and curated in silico tools. This high-quality genome sequencing effort has improved our understanding of chromosomal structure, gene set annotation, and lays the groundwork for identification of structural variants (e.g. transversions, translocations, and copy number variants), assessment of gene gain and loss, and comparison of transposable elements in future phylogenetic and population genomics studies.
(© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.)
Databáze: MEDLINE