Nitric oxide stimulates type IV MSHA pilus retraction in Vibrio cholerae via activation of the phosphodiesterase CdpA.

Autor:	Hughes HQ; Department of Biology, Indiana University, Bloomington, IN 47405., Floyd KA; Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064., Hossain S; Department of Chemistry, Stony Brook University, Stony Brook, NY 11794., Anantharaman S; Department of Chemistry, Stony Brook University, Stony Brook, NY 11794., Kysela DT; Department of Microbiology, Infectious Diseases, and Immunology, Université de Montréal, Montréal, QC H3T 1J4, Canada., Zöldi M; Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405., Barna L; Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405., Yu Y; Department of Chemistry, Indiana University, Bloomington, IN 47405., Kappler MP; Department of Chemistry, Indiana University, Bloomington, IN 47405., Dalia TN; Department of Biology, Indiana University, Bloomington, IN 47405., Podicheti RC; Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405., Rusch DB; Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405., Zhuang M; Department of Chemistry, University of Virginia, Charlottesville, VA 22904., Fraser CL; Department of Chemistry, University of Virginia, Charlottesville, VA 22904., Brun YV; Department of Microbiology, Infectious Diseases, and Immunology, Université de Montréal, Montréal, QC H3T 1J4, Canada., Jacobson SC; Department of Chemistry, Indiana University, Bloomington, IN 47405., McKinlay JB; Department of Biology, Indiana University, Bloomington, IN 47405., Yildiz FH; Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064., Boon EM; Department of Chemistry, Stony Brook University, Stony Brook, NY 11794.; Institute of Chemical Biology & Drug Discovery, Stony Brook University, Stony Brook, NY 11794., Dalia AB; Department of Biology, Indiana University, Bloomington, IN 47405; ankdalia@indiana.edu.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Feb 15; Vol. 119 (7).
DOI:	10.1073/pnas.2108349119
Abstrakt:	Bacteria use surface appendages called type IV pili to perform diverse activities including DNA uptake, twitching motility, and attachment to surfaces. The dynamic extension and retraction of pili are often required for these activities, but the stimuli that regulate these dynamics remain poorly characterized. To address this question, we study the bacterial pathogen Vibrio cholerae , which uses mannose-sensitive hemagglutinin (MSHA) pili to attach to surfaces in aquatic environments as the first step in biofilm formation. Here, we use a combination of genetic and cell biological approaches to describe a regulatory pathway that allows V. cholerae to rapidly abort biofilm formation. Specifically, we show that V. cholerae cells retract MSHA pili and detach from a surface in a diffusion-limited, enclosed environment. This response is dependent on the phosphodiesterase CdpA, which decreases intracellular levels of cyclic-di-GMP to induce MSHA pilus retraction. CdpA contains a putative nitric oxide (NO)-sensing NosP domain, and we demonstrate that NO is necessary and sufficient to stimulate CdpA-dependent detachment. Thus, we hypothesize that the endogenous production of NO (or an NO-like molecule) in V. cholerae stimulates the retraction of MSHA pili. These results extend our understanding of how environmental cues can be integrated into the complex regulatory pathways that control pilus dynamic activity and attachment in bacterial species. Competing Interests: The authors declare no competing interest. (Copyright © 2022 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
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