Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon.

Autor: Essone PN; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon. paulin.essone@cermel.org.; Institut für Tropenmedizin, Universität Tübingen and German Center for Infection Research Tübingen, Tübingen, Germany. paulin.essone@cermel.org.; Unité de Recherche et de Diagnostics Spécialisés, Laboratoire National de Santé Publique/Centre Hospitalier Universitaire Mère Enfant Fondation Jeanne EBORI, Libreville, Gabon. paulin.essone@cermel.org., Adegbite BR; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Institut für Tropenmedizin, Universität Tübingen and German Center for Infection Research Tübingen, Tübingen, Germany.; Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, location Amsterdam, Amsterdam Infection & Immunity, Amsterdam Public Health, University of Amsterdam, Amsterdam, The Netherlands., Mbadinga MJM; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon., Mbouna AV; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon., Lotola-Mougeni F; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon., Alabi A; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands., Edoa JR; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, location Amsterdam, Amsterdam Infection & Immunity, Amsterdam Public Health, University of Amsterdam, Amsterdam, The Netherlands., Lell B; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Division of Infectious Diseases and Tropical Medicine, Department of Medicine 1, Medical University of Vienna, Vienna, Austria., Alabi AS; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Institut für Tropenmedizin, Universität Tübingen and German Center for Infection Research Tübingen, Tübingen, Germany., Adegnika AA; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Institut für Tropenmedizin, Universität Tübingen and German Center for Infection Research Tübingen, Tübingen, Germany.; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands., Ramharter M; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine and Department of Internal Medicine I, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Siawaya JFD; Unité de Recherche et de Diagnostics Spécialisés, Laboratoire National de Santé Publique/Centre Hospitalier Universitaire Mère Enfant Fondation Jeanne EBORI, Libreville, Gabon., Grobusch MP; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Institut für Tropenmedizin, Universität Tübingen and German Center for Infection Research Tübingen, Tübingen, Germany.; Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, location Amsterdam, Amsterdam Infection & Immunity, Amsterdam Public Health, University of Amsterdam, Amsterdam, The Netherlands., Kremsner PG; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon.; Institut für Tropenmedizin, Universität Tübingen and German Center for Infection Research Tübingen, Tübingen, Germany., Agnandji ST; Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon. agnandjis@cermel.org.; Institut für Tropenmedizin, Universität Tübingen and German Center for Infection Research Tübingen, Tübingen, Germany. agnandjis@cermel.org.; The African Society of Clinical Trials and the African Congress for Clinical Trials, Lambaréné, Gabon. agnandjis@cermel.org.
Jazyk: angličtina
Zdroj: Infection [Infection] 2022 Aug; Vol. 50 (4), pp. 897-905. Date of Electronic Publication: 2022 Feb 08.
DOI: 10.1007/s15010-022-01760-8
Abstrakt: Background: The present study aimed to evaluate the diagnostic utility of creatine kinase-MB (CK-MB), hepcidin (HEPC), phospholipase A2 group IIA (PLa2G2A), and myosin-binding protein C (MYBPC1) for tuberculosis (TB). These four biomarkers are differentially regulated between quiescent Mycobacterium tuberculosis (Mtb) infected individuals (non-progressors to TB disease) and Mtb-infected TB disease progressors 6 months before the onset of symptoms.
Methods: We enrolled samples from patients experiencing moderate-to-severe pulmonary infections diseases including 23 TB cases confirmed by smear microscopy and culture, and 34 TB-negative cases. For each participant, the serum levels of the four biomarkers were measured using ELISA.
Results: The levels of CK-MB and HEPC were significantly reduced in patients with active TB disease. CK-MB median level was 2045 pg/ml (1455-4000 pg/ml) in active TB cases and 3245 pg/ml (1645-4000 pg/ml) in non-TB pulmonary diseases. Using the receiver operating characteristic curve (ROC) analysis, HEPC and CK-MB had the Area Under the Curve (AUC) of 79% (95% CI 67-91%) and 81% (95% CI 69-93%), respectively. Both markers correlated with TB diagnosis as a single marker. PLa2G2A and MYBPC1 with AUCs of 48% (95% CI 36-65%) and 62% (95% CI 48-76%) did not performed well as single biomarkers. The three markers'model (CK-MB-HEPC-PLa2G2A) had the highest diagnostic accuracy at 82% (95% CI 56-82%) after cross-validation.
Conclusion: CK-MB and HEPC levels were statistically different between confirmed TB cases and non-TB cases. This study yields promising results for the rapid diagnosis of TB disease using a single marker or three biomarkers model.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz: