OsEIL1 protects rice growth under NH 4 + nutrition by regulating OsVTC1-3-dependent N-glycosylation and root NH 4 + efflux.

Autor: Li G; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China., Zhang L; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China., Wu J; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.; University of the Chinese Academy of Sciences, Beijing, China., Yue X; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China., Wang M; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China., Sun L; School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia., Di D; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China., Kronzucker HJ; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.; Faculty of Land and Food Systems, University of British Columbia, Vancouver, British Columbia, Canada., Shi W; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2022 May; Vol. 45 (5), pp. 1537-1553. Date of Electronic Publication: 2022 Feb 17.
DOI: 10.1111/pce.14283
Abstrakt: Rice is known for its superior adaptation to ammonium (NH 4 + ) as a nitrogen source. Compared to many other cereals, it displays lower NH 4 + efflux in roots and higher nitrogen-use efficiency on NH 4 + . A critical role for GDP-mannose pyrophosphorylase (VTC1) in controlling root NH 4 + fluxes was previously documented in Arabidopsis, but the molecular pathways involved in regulating VTC1-dependent NH 4 + efflux remain unclear. Here, we report that ETHYLENE-INSENSITIVE3-LIKE1 (OsEIL1) acts as a key transcription factor regulating OsVTC1-3-dependent NH 4 + efflux and protein N-glycosylation in rice grown under NH 4 + nutrition. We show that OsEIL1 in rice plays a contrasting role to Arabidopsis-homologous ETHYLENE-INSENSITIVE3 (AtEIN3) and maintains rice growth under NH 4 + by stabilizing protein N-glycosylation and reducing root NH 4 + efflux. OsEIL1 constrains NH 4 + efflux by activation of OsVTC1-3, but not OsVTC1-1 or OsVTC1-8. OsEIL1 binds directly to the promoter EIN3-binding site (EBS) of OsVTC1-3 in vitro and in vivo and acts to increase the transcription of OsVTC1-3. Our work demonstrates an important link between excessive root NH 4 + efflux and OsVTC1-3-mediated protein N-glycosylation in rice grown under NH 4 + nutrition and identifies OsEIL1 as a direct genetic regulator of OsVTC1-3 expression.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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