Pseudomonas Aeruginosa Theft Biofilm Require Host Lipids of Cutaneous Wound.

Autor:	Sinha M; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN., Ghosh N; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN., Wijesinghe DS; Department of Pharmacotherapy, and Outcomes Science, School of Pharmacy, Virginia Commonwealth University, Richmond, VA., Mathew-Steiner SS; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN., Das A; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN., Singh K; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN., El Masry M; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN.; Department of Plastic and Reconstructive Surgery, Zagazig University, Egypt., Khanna S; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN., Inoue H; Institute of Microbial Chemistry, Microbial, Chemistry Research Foundation, Tokyo, Japan., Yamazaki K; Institute of Microbial Chemistry, Microbial, Chemistry Research Foundation, Tokyo, Japan., Kawada M; Institute of Microbial Chemistry, Microbial, Chemistry Research Foundation, Tokyo, Japan., Gordillo GM; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN., Roy S; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN., Sen CK; Indiana Center for Regenerative Medicine and Engineering, Department, of Surgery, IU Heath Comprehensive Wound Center, Indiana University, School of Medicine, Indianapolis, IN.
Jazyk:	angličtina
Zdroj:	Annals of surgery [Ann Surg] 2023 Mar 01; Vol. 277 (3), pp. e634-e647. Date of Electronic Publication: 2021 Oct 12.
DOI:	10.1097/SLA.0000000000005252
Abstrakt:	Objective: This work addressing complexities in wound infection, seeks to test the reliance of bacterial pathogen Pseudomonas aeruginosa (PA) on host skin lipids to form biofilm with pathological consequences. Background: PA biofilm causes wound chronicity. Both CDC as well as NIH recognizes biofilm infection as a threat leading to wound chronicity. Chronic wounds on lower extremities often lead to surgical limb amputation. Methods: An established preclinical porcine chronic wound biofilm model, infected with PA or Pseudomonas aeruginosa ceramidase mutant (PA ∆Cer ), was used. Results: We observed that bacteria drew resource from host lipids to induce PA ceramidase expression by three orders of magnitude. PA utilized product of host ceramide catabolism to augment transcription of PA ceramidase. Biofilm formation was more robust in PA compared to PA ∆Cer . Downstream products of such metabolism such as sphingosine and sphingosine-1-phosphate were both directly implicated in the induction of ceramidase and inhibition of peroxisome proliferator-activated receptor (PPAR)δ, respectively. PA biofilm, in a ceram-idastin-sensitive manner, also silenced PPARδ via induction of miR-106b. Low PPARδ limited ABCA12 expression resulting in disruption of skin lipid homeostasis. Barrier function of the wound-site was thus compromised. Conclusions: This work demonstrates that microbial pathogens must co-opt host skin lipids to unleash biofilm pathogenicity. Anti-biofilm strategies must not necessarily always target the microbe and targeting host lipids at risk of infection could be productive. This work may be viewed as a first step, laying fundamental mechanistic groundwork, toward a paradigm change in biofilm management. Competing Interests: The authors report no conflict of interest. (Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc.)
Databáze:	MEDLINE
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