| Autor: |
Pandey A; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Malla WA; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Sahu AR; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Wani SA; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Khan RIN; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Saxena S; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Ramteke PW; Department of Biological Sciences, SHUATS, Allahabad, India., Praharaj MR; Genomics and Bioinformatics, National Institute of Animal Biotechnology, Hyderabad, India., Kumar A; Division of Animal Genetics and Breeding, ICAR-IVRI, Bareilly, India., Rajak KK; Division of Biological Products, ICAR-IVRI, Bareilly, India., Mishra B; Division of Biological Products, ICAR-IVRI, Bareilly, India., Muthuchelvan D; Division of Virology, ICAR-IVRI, Nainital, India., Sajjanar B; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Mishra BP; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Singh RK; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India., Gandham RK; Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India.; Genomics and Bioinformatics, National Institute of Animal Biotechnology, Hyderabad, India. |
| Abstrakt: |
Peste des petits ruminants (PPR) characterized by fever, sore mouth, conjunctivitis, gastroenteritis, and pneumonia, is an acute, highly contagious viral disease of sheep and goats. The role of long non-coding RNAs (lncRNAs) in PPRV infection has not been explored to date. In this study, the transcriptome profiles of virulent Peste des petits ruminants virus (PPRV) infected goat tissues - lung and spleen were analyzed to identify the role of lncRNAs in PPRV infection. A total of 13,928 lncRNA transcripts were identified, out of which 170 were known lncRNAs. Intergenic lncRNAs (7625) formed the major chunk of the novel lncRNA transcripts. Differential expression analysis revealed that 15 lncRNAs (11 downregulated and 4 upregulated) in the PPRV infected spleen samples and 16 lncRNAs (13 downregulated and 3 upregulated) in PPRV infected lung samples were differentially expressed as compared to control. The differentially expressed lncRNAs (DElncRNAs) possibly regulate various immunological processes related to natural killer cell activation, antigen processing and presentation, and B cell activity, by regulating the expression of mRNAs through the cis- or trans-regulatory mechanism. Functional enrichment analysis of differentially expressed mRNAs (DEmRNAs) revealed enrichment of immune pathways and biological processes in concordance with the pathways in which correlated lncRNA-neighboring genes were enriched. The results suggest that a coordinated immune response is raised in both lung and spleen tissues of the goat through mRNA-lncRNA crosstalk. |
