The metabolism of valerylfentanyl using human liver microsomes and zebrafish larvae.

Autor: Cooman T; Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA., Hoover B; Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA., Sauvé B; Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA., Bergeron SA; Department of Biology, West Virginia University, Morgantown, West Virginia, USA., Quinete N; Institute of Environment and Department of Chemistry and Biochemistry, Florida International University, Miami, Florida, USA., Gardinali P; Institute of Environment and Department of Chemistry and Biochemistry, Florida International University, Miami, Florida, USA., Arroyo LE; Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA.
Jazyk: angličtina
Zdroj: Drug testing and analysis [Drug Test Anal] 2022 Jun; Vol. 14 (6), pp. 1116-1129. Date of Electronic Publication: 2022 Feb 12.
DOI: 10.1002/dta.3233
Abstrakt: Valerylfentanyl, a novel synthetic opioid less potent than fentanyl, has been reported in biological samples, but there are limited studies on its pharmacokinetic properties. The goal of this study was to elucidate the metabolism of valerylfentanyl using an in vitro human liver microsome (HLM) model compared with an in vivo zebrafish model. Nineteen metabolites were detected with N-dealkylation-valeryl norfentanyl and hydroxylation as the major metabolic pathways. The major metabolites in HLMs were also detected in 30 day postfertilization zebrafish. An authentic liver specimen that tested positive for valerylfentanyl, among other opioids and stimulants, revealed the presence of a metabolite that shared transitions and retention time as the hydroxylated metabolite of valerylfentanyl but could not be confirmed without an authentic standard. 4-Anilino-N-phenethylpiperidine (4-ANPP), a common metabolite to other fentanyl analogs, was also detected. In this study, we elucidated the metabolic pathway of valerylfentanyl, confirmed two metabolites using standards, and demonstrated that the zebrafish model produced similar metabolites to the HLM model for opioids.
(© 2022 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
Externí odkaz: