Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production.

Autor: van Aalst ACA; Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the Netherlands., de Valk SC; Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the Netherlands., van Gulik WM; Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the Netherlands., Jansen MLA; DSM Biotechnology Centre, Alexander Fleminglaan 1, 2613, AX Delft, the Netherlands., Pronk JT; Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the Netherlands., Mans R; Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ Delft, the Netherlands.
Jazyk: angličtina
Zdroj: Synthetic and systems biotechnology [Synth Syst Biotechnol] 2022 Jan 22; Vol. 7 (1), pp. 554-566. Date of Electronic Publication: 2022 Jan 22 (Print Publication: 2022).
DOI: 10.1016/j.synbio.2021.12.010
Abstrakt: Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae . This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.
Competing Interests: The PhD project of AA is funded by DSM Bio-based Products & Services B.V. (Delft, The Netherlands). Royal DSM owns intellectual property rights of technology discussed in this paper.
(© 2022 The Authors.)
Databáze: MEDLINE
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