Efficacy of the incorporation between self-encapsulation and cryoprotectants on improving the freeze-dried survival of probiotic bacteria.

Autor: Nguyen TT; Hutech Institute of Applied Science, HUTECH University, Ho Chi Minh City, Vietnam., Nguyen PT; Graduate University of Sciences and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.; Department of Biotechnology, An Giang University, An Giang, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Nguyen TT; Department of Biotechnology, Can Tho Science and Technology Application Center, Can Tho City, Vietnam., Nguyen TB; Department of Biotechnology, Can Tho Science and Technology Application Center, Can Tho City, Vietnam., Bui NB; Department of Biotechnology, Can Tho Science and Technology Application Center, Can Tho City, Vietnam., Nguyen HT; Department of Biotechnology, An Giang University, An Giang, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2022 Apr; Vol. 132 (4), pp. 3217-3225. Date of Electronic Publication: 2022 Feb 16.
DOI: 10.1111/jam.15473
Abstrakt: Aims: This study aimed to improve the viability of probiotic bacteria during freeze-drying by the combination of self-encapsulation and cryoprotectants.
Methods and Results: Lactiplantibacillus plantarum VAL6 and Lactobacillus acidophilus VAR1 were exposed to environmental stresses including temperature, pH and increased CO 2 concentration before performing freeze-drying with the addition of cryoprotectants. The results proved that tested stresses can stimulate the bacteria to synthesize more extracellular polymeric substances to form self-encapsulation that increases their freeze-dried viability. In combination with cryoprotectants to form double-layered microencapsulation, L. plantarum VAL6 stressed at pH 3.5 in combination with whey protein isolate could achieve the highest Improving Cell Viability of 4361-fold, while L. acidophilus VAR1 stressed at 25 o C in combination with alginate gave a maximum Improving Cell Viability of 73.33-fold.
Conclusions: The combination of self-encapsulation and cryoprotectants significantly improves the freeze-dried viability of probiotics.
Significance and Impact of the Study: This is the first report that uses environmental stress to stimulate extracellular polymeric substance synthesis for self-encapsulation formation combined with the addition of cryoprotectants to enhance the freeze-dried survival of probiotics. This could be a novel approach in improving the viability of probiotic strains for various applications.
(© 2022 Society for Applied Microbiology.)
Databáze: MEDLINE
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