Early decrease in Cx36 is associated with increased cell adhesion molecules (CAMs) junctional content in mouse pancreatic islets after short-term high-fat diet feeding.

Autor: Martinez C; Department of Biosciences, Federal University of São Paulo, Santos, SP, Brazil., Maschio DA; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil., de Fontes CC; Department of Biosciences, Federal University of São Paulo, Santos, SP, Brazil., Vanzela EC; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil., Benfato ID; Department of Biosciences, Federal University of São Paulo, Santos, SP, Brazil., Gazarini ML; Department of Biosciences, Federal University of São Paulo, Santos, SP, Brazil., Carneiro EM; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil., de Oliveira CAM; Department of Biosciences, Federal University of São Paulo, Santos, SP, Brazil., Collares-Buzato CB; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil., Carvalho CPF; Department of Biosciences, Federal University of São Paulo, Santos, SP, Brazil. Electronic address: ccarvalho13@unifesp.br.
Jazyk: angličtina
Zdroj: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft [Ann Anat] 2022 Apr; Vol. 241, pp. 151891. Date of Electronic Publication: 2022 Jan 31.
DOI: 10.1016/j.aanat.2022.151891
Abstrakt: Cell-to-cell interactions mediated by intercellular junctions (IJs) are crucial for beta-cell functioning and proper insulin secretion, however, their role in type-2 diabetes is still unclear. This work aimed to evaluate the cellular distribution and expression of proteins associated with adherens (AJs) and gap junctions (GJs) in pancreatic islets of C57BL6 mice fed a high-fat (HF) diet. The administration of HF diet for 30 days induced an increase in body weight, post-prandial glycemia, insulinemia, glucose intolerance, and moderate insulin resistance associated with mild perturbations in insulin secretion. The intercellular content of the AJ-associated proteins (namely, E-, N-cadherins, and α-, β-catenins) was significantly higher in islet cells of HF-fed mice. Inversely, the gap junctional content of Cx36 was significantly decreased, as revealed by immunofluorescence, which was paralleled by a reduction in the frequency of calcium oscillations in islets of prediabetic mice. In conclusion, the endocrine pancreas displays significant changes in the content of several junctional proteins at the cell-cell contact region following short-term HF diet administration, indicating that IJs may be involved in the adaptive response of beta cells seen during this state.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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