Anti-Trichomonas vaginalis activity and chemical analysis of metabolites produced by marine-associated fungi.

Autor: Senger FR; Faculdade de Farmácia, Universidade Federal Do Rio Grande Do Sul, Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil.; Centro de Biotecnologia Do Estado de Rio Grande Do Sul, Universidade Federal Do Rio Grande Do Sul, Porto AlegrePorto Alegre, RS, Brazil., Campos-Silva R; Faculdade de Farmácia, Universidade Federal Do Rio Grande Do Sul, Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil.; Centro de Biotecnologia Do Estado de Rio Grande Do Sul, Universidade Federal Do Rio Grande Do Sul, Porto AlegrePorto Alegre, RS, Brazil., Landell MF; Instituto de Ciências Biológicas E da Saúde, Universidade Federal de Alagoas, Maceió, AL, Brazil., Silva DB; Laboratório de Produtos Naturais E Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos E Nutrição (FACFAN), Universidade Federal de Mato Grosso Do Sul (UFMS), Campo Grande, MS, Brazil., Menezes CB; Faculdade de Farmácia, Universidade Federal Do Rio Grande Do Sul, Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil., Rigo GV; Faculdade de Farmácia, Universidade Federal Do Rio Grande Do Sul, Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil.; Centro de Biotecnologia Do Estado de Rio Grande Do Sul, Universidade Federal Do Rio Grande Do Sul, Porto AlegrePorto Alegre, RS, Brazil., Silva LN; Faculdade de Farmácia, Universidade Federal Do Rio Grande Do Sul, Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil.; Centro de Biotecnologia Do Estado de Rio Grande Do Sul, Universidade Federal Do Rio Grande Do Sul, Porto AlegrePorto Alegre, RS, Brazil., Trentin DS; Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil., Macedo AJ; Faculdade de Farmácia, Universidade Federal Do Rio Grande Do Sul, Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil.; Centro de Biotecnologia Do Estado de Rio Grande Do Sul, Universidade Federal Do Rio Grande Do Sul, Porto AlegrePorto Alegre, RS, Brazil., Tasca T; Faculdade de Farmácia, Universidade Federal Do Rio Grande Do Sul, Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil. tiana.tasca@ufrgs.br.; Centro de Biotecnologia Do Estado de Rio Grande Do Sul, Universidade Federal Do Rio Grande Do Sul, Porto AlegrePorto Alegre, RS, Brazil. tiana.tasca@ufrgs.br.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2022 Mar; Vol. 121 (3), pp. 981-989. Date of Electronic Publication: 2022 Feb 03.
DOI: 10.1007/s00436-022-07442-6
Abstrakt: Trichomoniasis is the most common non-viral sexually transmitted infection worldwide and it may have serious consequences, especially for women. Currently, 5-nitroimidazole drugs are the treatment of choice for trichomoniasis, although presenting adverse effects and reported cases of drug resistance. Metabolites isolated from marine fungi have attracted considerable attention due to their unique chemical structures with diverse biological activities, including antiprotozoal activity. In this study, we showed the anti-Trichomonas vaginalis activity of fractions obtained from marine fungi and the chemical composition of the most active fraction was determined. Ethyl acetate fractions of the fungus Aspergillus niger (EAE03) and Trichoderma harzianum/Hypocrea lixii complex (EAE09) were active against T. vaginalis. These samples, EAE03 and EAE09, were also effective against the fresh clinical isolate metronidazole-resistant TV-LACM2R, presenting MIC values of 2.0 mg/mL and 1.0 mg/mL, respectively. The same MIC values were found against ATCC 30,236 T. vaginalis isolate. In vitro cytotoxicity revealed only the fraction named EAE03 with no cytotoxic effect; however, the active fractions did not promote a significant hemolytic effect after 1-h incubation. Already, the in vivo toxicity evaluation using Galleria mellonella larvae demonstrated that none of the tested samples caused a reduction in animal survival. The fraction EAE03 was followed for purification steps and analyzed by LC-DAD-MS. Eleven compounds were annotated, including butyrolactone, butanolide, and atromentin. Overall, the range of activities reported confirms the potential of marine fungi to produce bioactive molecules.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: