TanP: A Multifunctional Anionic Peptide From Tityus stigmurus Scorpion Venom.

Autor:	de Melo MMA; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Brazil., Oliveira VDS; Laboratory of Coordination Chemistry and Polymers, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal, Brazil., de Queiroz Neto MF; Laboratory of Natural Polymer Biotechnology, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, Brazil., Paiva WS; Laboratory of Natural Polymer Biotechnology, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, Brazil., Torres-Rêgo M; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Brazil.; Laboratory of Synthesis and Isolation of Organic Compounds, Chemistry Institute, Federal University of Rio Grande do Norte, Natal, Brazil., Silva SRB; Institute of Brain, Federal University of Rio Grande do Norte, Natal, Brazil., Pontes DL; Laboratory of Coordination Chemistry and Polymers, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal, Brazil., Rocha HAO; Laboratory of Natural Polymer Biotechnology, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, Brazil., de Souza MÂF; Laboratory of Computational Chemistry, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal, Brazil., da Silva-Júnior AA; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Brazil., Fernandes-Pedrosa MF; Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in molecular biosciences [Front Mol Biosci] 2022 Jan 17; Vol. 8, pp. 785316. Date of Electronic Publication: 2022 Jan 17 (Print Publication: 2021).
DOI:	10.3389/fmolb.2021.785316
Abstrakt:	Anionic peptides of scorpions are molecules rich in aspartic and/or glutamic acid residues and correspond to a class of peptides without disulfide bonds that are still little explored. TanP is a linear anionic peptide (50 amino acid residues and net charge -20) present in the venom gland of the scorpion, Tityus stigmurus , with chelating properties for Cu 2+ ion and immunomodulatory properties. The therapeutic application of chelating molecules is related to cases of acute or chronic intoxication by metals, neurodegenerative diseases, hematological diseases, healing of skin wounds, cardiovascular diseases, and cancer. In this approach, the chelating activity of TanP was evaluated in relation to new metal ions (Fe 2+ and Zn 2+ ) of biological importance, as well as its antioxidant, hemostatic, immunomodulatory, and healing potential, aiming to expand the biological and biotechnological potential of this peptide. TanP (25 µM) was able to form stable complexes with Fe 2+ in a ratio of 1:5 (TanP: Fe 2+ ). Theoretical results suggest that TanP can work as a sensor to identify and quantify Fe 2+ ions. The fluorescence intensity of TanP (1.12 µM) decreased significantly after the addition of Fe 2+ , obtaining the highest ratio 1: 7.4 (TanP: Fe 2+ ) that led to the lowest fluorescence intensity. For Zn 2+ , no relevant spectral change was noted. TanP (50 µM) showed a maximum of 3% of hemolytic activity, demonstrating biocompatibility, as well as exhibiting a 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity of above 70% at all the concentrations tested (1-25 μM), and 89.7% iron-chelating activity at 25 μM and 96% hydroxyl radical-scavenging activity at 73.6 μM. In addition, TanP (12.5 and 25 µM) revealed an anticoagulant effect, prolonging the clotting time in prothrombin time and activated partial thromboplastin time assays, with no fibrinogenolytic activity. TanP (12.5 and 25 µM) induced the release of TNF-α by murine macrophages, in the absence of lipopolysaccharides, with a concentration-dependent increase and also stimulated the migration of 3T3 cells in the in vitro healing assay. Thus, TanP revealed a multifunctional potential, being useful as a prototype for the development of new therapeutic and biotechnological agents. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Melo, Oliveira, Queiroz Neto, Paiva, Torres-Rêgo, Silva, Pontes, Rocha, Souza, Silva-Júnior and Fernandes-Pedrosa.)
Databáze:	MEDLINE
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