Mutations in JAK/STAT and NOTCH1 Genes Are Enriched in Post-Transplant Lymphoproliferative Disorders.

Autor:	Butzmann A; Agilent Technologies, Santa Clara, CA, United States.; Department of Pathology, University of California, San Francisco, San Francisco, CA, United States., Sridhar K; Department of Pathology, University of California, San Francisco, San Francisco, CA, United States., Jangam D; Department of Pathology, Stanford University, Stanford, CA, United States., Song H; Department of Pathology, University of California, San Francisco, San Francisco, CA, United States., Singh A; Department of Pathology, Stanford University, Stanford, CA, United States., Kumar J; Department of Pathology, Stanford University, Stanford, CA, United States., Chisholm KM; Department of Laboratories, Seattle Children's Hospital, Seattle, WA, United States., Pinsky B; Department of Pathology, Stanford University, Stanford, CA, United States., Huang F; Department of Pathology, University of California, San Francisco, San Francisco, CA, United States., Ohgami RS; Department of Pathology, University of California, San Francisco, San Francisco, CA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in oncology [Front Oncol] 2022 Jan 17; Vol. 11, pp. 790481. Date of Electronic Publication: 2022 Jan 17 (Print Publication: 2021).
DOI:	10.3389/fonc.2021.790481
Abstrakt:	Post-transplant lymphoproliferative disorders (PTLD) are diseases occurring in immunocompromised patients after hematopoietic stem cell transplantation (HCT) or solid organ transplantation (SOT). Although PTLD occurs rarely, it may be associated with poor outcomes. In most cases, PTLD is driven by Epstein-Barr virus (EBV) infection. Few studies have investigated the mutational landscape and gene expression profile of PTLD. In our study, we performed targeted deep sequencing and RNA-sequencing (RNA-Seq) on 16 cases of florid follicular hyperplasia (FFH) type PTLD and 15 cases of other PTLD types that include: ten monomorphic (M-PTLD), three polymorphic (P-PTLD), and two classic Hodgkin lymphoma type PTLDs (CHL-PTLD). Our study identified recurrent mutations in JAK3 in five of 15 PTLD cases and one of 16 FFH-PTLD cases, as well as 16 other genes that were mutated in M-PTLD, P-PTLD, CHL-PTLD and FFH-PTLD. Digital image analysis demonstrated significant differences in single cell area, major axis, and diameter when comparing cases of M-PTLD and P-PTLD to FFH-PTLD. No morphometric relationship was identified with regards to a specific genetic mutation. Our findings suggest that immune regulatory pathways play an essential role in PTLD, with the JAK / STAT pathway affected in many PTLDs. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Butzmann, Sridhar, Jangam, Song, Singh, Kumar, Chisholm, Pinsky, Huang and Ohgami.)
Databáze:	MEDLINE
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