Downregulation of metallothionein 2A reduces migration, invasion and proliferation activities in human squamous cell carcinoma cells.

Autor: Dias AM; Department of Oral Pathology, School of Dentistry, Universidade Federal do Para, Avenida Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil., de Mendonça RP; Department of Oral Pathology, School of Dentistry, Universidade Federal do Para, Avenida Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil., da Silva Kataoka MS; Department of Oral Pathology, School of Dentistry, Universidade Federal do Para, Avenida Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil., Jaeger RG; Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes 1524, Sao Paulo, 05508-000, Brazil., de Jesus Viana Pinheiro J; Department of Oral Pathology, School of Dentistry, Universidade Federal do Para, Avenida Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil. radface@hotmail.com., de Melo Alves Junior S; Department of Oral Pathology, School of Dentistry, Universidade Federal do Para, Avenida Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2022 May; Vol. 49 (5), pp. 3665-3674. Date of Electronic Publication: 2022 Feb 02.
DOI: 10.1007/s11033-022-07206-6
Abstrakt: Background: The invasive behaviour of squamous cell carcinoma (SCC), a common malignant tumour of the mouth, is a process mediated by cell proliferation, extracellular matrix proteolysis and other factors. Studies have shown a potential relationship between growth factors, metallothionein 2A (MT2A) and matrix metalloproteinase (MMP) activation in malignant tumours. The aim of this study was to downregulate MT2A in cells (Cal27) derived from human squamous cell carcinoma.
Methods: Cal27 cells with reduced MT2A were subjected to proliferation, migration and invasion assays. Immunofluorescence and western blot confirmed MT2A depletion by siRNA. Growth curve assays assessed cell proliferation. Indirect immunofluorescence analysed the expression of MT2A, MMP-2, MMP-9, epidermal growth factor (EGF), transforming growth factor alpha (TGF-α), tumour necrosis factor alpha (TNF-α) and Ki67. Zymography evaluated the effects of MT2A silencing on MMP-2 and -9 expression. Migration and invasion activities were evaluated using migration and invasion assays.
Results: CAL27 cells displayed MT2A, MMP-2, MMP-9, EGF, TGF-α, TNF-α and Ki67. MT2A depletion decreased MMP-9, EGF, TGF-α and Ki67 protein levels, while increasing TNF-α.
Conclusions: MT2A downregulation reduced cell proliferation, migration and invasion activities. Therefore, MT2A has an important role in cell proliferation, migration and invasion in human oral SCC cells.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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