Membrane adaptation in the hyperthermophilic archaeon Pyrococcus furiosus relies upon a novel strategy involving glycerol monoalkyl glycerol tetraether lipids.

Autor: Tourte M; Univ Lyon, Univ. Lyon 1, CNRS, UMR 5240, Villeurbanne, F-69622, France.; Univ Lyon, INSA Lyon, CNRS, UMR 5240, Villeurbanne, F-69621, France., Schaeffer P; Univ. Strasbourg, CNRS, UMR 7177, Strasbourg, F-67000, France., Grossi V; Univ Lyon, Univ. Lyon 1, CNRS, ENSL, UJM, UMR 5276 LGL-TPE, Villeurbanne, F-69622, France., Oger PM; Univ Lyon, INSA Lyon, CNRS, UMR 5240, Villeurbanne, F-69621, France.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2022 Apr; Vol. 24 (4), pp. 2029-2046. Date of Electronic Publication: 2022 Mar 11.
DOI: 10.1111/1462-2920.15923
Abstrakt: Microbes preserve membrane functionality under fluctuating environmental conditions by modulating their membrane lipid composition. Although several studies have documented membrane adaptations in Archaea, the influence of most biotic and abiotic factors on archaeal lipid compositions remains underexplored. Here, we studied the influence of temperature, pH, salinity, the presence/absence of elemental sulfur, the carbon source and the genetic background on the lipid core composition of the hyperthermophilic neutrophilic marine archaeon Pyrococcus furiosus. Every growth parameter tested affected the lipid core composition to some extent, the carbon source and the genetic background having the greatest influence. Surprisingly, P. furiosus appeared to only marginally rely on the two major responses implemented by Archaea, i.e. the regulation of the ratio of diether to tetraether lipids and that of the number of cyclopentane rings in tetraethers. Instead, this species increased the ratio of glycerol monoalkyl glycerol tetraethers (GMGT, aka. H-shaped tetraethers) to glycerol dialkyl glycerol tetraethers in response to decreasing temperature and pH and increasing salinity, thus providing for the first time evidence of adaptive functions for GMGT. Besides P. furiosus, numerous other species synthesize significant proportions of GMGT, which suggests that this unprecedented adaptive strategy might be common in Archaea.
(© 2022 Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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