Cultivation and biogeochemical analyses reveal insights into methanogenesis in deep subseafloor sediment at a biogenic gas hydrate site.

Autor: Katayama T; Research Institute for Geo-Resources and Environment, Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan., Yoshioka H; Research Institute for Geo-Resources and Environment, Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan. hi-yoshioka@aist.go.jp., Kaneko M; Research Institute for Geo-Resources and Environment, Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan., Amo M; Technology and Research Center, Japan Oil, Gas and Metals National Corporation, Chiba, Japan., Fujii T; Technology and Research Center, Japan Oil, Gas and Metals National Corporation, Chiba, Japan.; Oil & Gas Upstream Business Unit, Japan Oil, Gas and Metals National Corporation, Tokyo, Japan., Takahashi HA; Research Institute of Earthquake and Volcano Geology, Geological Survey of Japan, AIST, Tsukuba, Japan., Yoshida S; National Institutes for Quantum Science and Technology, Chiba, Japan.; Institute for Environmental Sciences, Aomori, Japan., Sakata S; Research Institute for Geo-Resources and Environment, Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Jazyk: angličtina
Zdroj: The ISME journal [ISME J] 2022 May; Vol. 16 (5), pp. 1464-1472. Date of Electronic Publication: 2022 Feb 02.
DOI: 10.1038/s41396-021-01175-7
Abstrakt: Gas hydrates deposited in subseafloor sediments are considered to primarily consist of biogenic methane. However, little evidence for the occurrence of living methanogens in subseafloor sediments has been provided. This study investigated viable methanogen diversity, population, physiology and potential activity in hydrate-bearing sediments (1-307 m below the seafloor) from the eastern Nankai Trough. Radiotracer experiments, the quantification of coenzyme F430 and molecular sequencing analysis indicated the occurrence of potential methanogenic activity and living methanogens in the sediments and the predominance of hydrogenotrophic methanogens followed by methylotrophic methanogens. Ten isolates and nine representative culture clones of hydrogenotrophic, methylotrophic and acetoclastic methanogens were obtained from the batch incubation of sediments and accounted for 0.5-76% of the total methanogenic sequences directly recovered from each sediment. The hydrogenotrophic methanogen isolates of Methanocalculus and Methanoculleus that dominated the sediment methanogen communities produced methane at temperatures from 4 to 55 °C, with an abrupt decline in the methane production rate at temperatures above 40 °C, which is consistent with the depth profiles of potential methanogenic activity in the Nankai Trough sediments in this and previous studies. Our results reveal the previously overlooked phylogenetic and metabolic diversity of living methanogens, including methylotrophic methanogenesis.
(© 2021. The Author(s).)
Databáze: MEDLINE
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