Ecdysterone prevents negative effect of acute immobilization stress on energy metabolism of rat liver mitochondria.

Autor: Baev AY; Laboratory of Experimental Biophysics, Centre for Advanced Technologies, Tashkent, Uzbekistan; Department of Biophysics, Faculty of Biology, National University of Uzbekistan, Tashkent, Uzbekistan. Electronic address: baev.a.yu@gmail.com., Charishnikova OS; Laboratory of Experimental Biophysics, Centre for Advanced Technologies, Tashkent, Uzbekistan., Khasanov FA; Laboratory of Experimental Biophysics, Centre for Advanced Technologies, Tashkent, Uzbekistan; Department of Biochemistry, Faculty of Biology, National University of Uzbekistan, Tashkent, Uzbekistan., Nebesnaya KS; Laboratory of Experimental Biophysics, Centre for Advanced Technologies, Tashkent, Uzbekistan; Department of Biophysics, Faculty of Biology, National University of Uzbekistan, Tashkent, Uzbekistan., Makhmudov AR; Laboratory of Experimental Biophysics, Centre for Advanced Technologies, Tashkent, Uzbekistan; Faculty of Chemistry, National University of Uzbekistan, Tashkent, Uzbekistan., Rakhmedova MT; Laboratory of Experimental Biophysics, Centre for Advanced Technologies, Tashkent, Uzbekistan., Khushbaktova ZA; S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan., Syrov VN; S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan., Levitskaya YV; Laboratory of Experimental Biophysics, Centre for Advanced Technologies, Tashkent, Uzbekistan; Department of Biophysics, Faculty of Biology, National University of Uzbekistan, Tashkent, Uzbekistan. Electronic address: yv.levickaya@gmail.com.
Jazyk: angličtina
Zdroj: The Journal of steroid biochemistry and molecular biology [J Steroid Biochem Mol Biol] 2022 May; Vol. 219, pp. 106066. Date of Electronic Publication: 2022 Jan 29.
DOI: 10.1016/j.jsbmb.2022.106066
Abstrakt: Ecdysterone is a naturally occurring steroid hormone, which presents in arthropods and in a number of plants as an insect defence tool. There are many studies showing that application of ecdysterone can alter mitochondrial functions of mammalian cells, however it is not clear whether its effects are direct or mediated by activation of other cellular processes. In our study, we have shown how ecdysterone acts at the mitochondrial level in normal conditions and in certain pathology. We have demonstrated that application of immobilization stress to male rats causes uncoupling of mitochondrial oxidative phosphorylation, the preliminary application of ecdysterone prevents negative effect of immobilization stress on mitochondria. In-vitro experiments with isolated mitochondria have shown that ecdysterone can increase mitochondrial coupling and hyperpolarise mitochondria but without a noticeable effect on ADP/O ratio. Molecular docking experiments revealed that ecdysterone has high binding energy with mitochondrial F O F 1 ATP synthase, but further biochemical analysis have not revealed either stimulatory or inhibitory effect of ecdysterone on F O F 1 ATPase activity of the enzyme. Thus, ecdysterone can directly affect mitochondrial bioenergetics, though we assume that its preventive effect on mitochondria during immobilization stress is also coupled with the activation of some other cellular processes.
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Databáze: MEDLINE
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