High-Resolution ATAC-Seq Analysis of Frozen Clinical Tissues.

Autor:	Cejas P; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA. paloma_cejas@dfci.harvard.edu.; Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA, USA. paloma_cejas@dfci.harvard.edu.; Translational Oncology Laboratory, Hospital La Paz Institute for Health Research (IdiPAZ) and CIBERONC, La Paz University Hospital, Madrid, Spain. paloma_cejas@dfci.harvard.edu., Long HW; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA. Henry_Long@dfci.harvard.edu.; Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA, USA. Henry_Long@dfci.harvard.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2458, pp. 259-267.
DOI:	10.1007/978-1-0716-2140-0_14
Abstrakt:	The ATAC-seq method enables the genome-wide analysis of accessible chromatin revealing transcriptionally active and poised regulatory elements. The ATAC-seq analysis of clinical specimens at a single-cell resolution reveals the cellular composition of the tissue contributing to the understanding of intra-tissue heterogeneity. Here we describe our method for nuclei isolation from frozen specimens with wide applicability across tissue types, producing nuclei suitable for a number of molecular profiling methods including ATAC-seq in bulk and at a single-cell resolution. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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