Design of an elastic porous injectable biomaterial for tissue regeneration and volume retention.

Autor: Béduer A; Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CMU, Rue Michel-Servet 1, 1211 Genève 4, Switzerland; Volumina-Medical SA, Route de la Corniche 5, CH-1066 Epalinges, Switzerland., Genta M; Laboratory of Microsystems 4, STI-IMT, Station 17, EPFL, 1015 Lausanne, Switzerland., Kunz N; Center for Biomedical Imaging CIBM, ENT-R, Station 6, EPFL, 1015 Lausanne, Switzerland., Verheyen C; Laboratory of Microsystems 4, STI-IMT, Station 17, EPFL, 1015 Lausanne, Switzerland., Martins M; Volumina-Medical SA, Route de la Corniche 5, CH-1066 Epalinges, Switzerland; Laboratory of Microsystems 4, STI-IMT, Station 17, EPFL, 1015 Lausanne, Switzerland., Brefie-Guth J; Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CMU, Rue Michel-Servet 1, 1211 Genève 4, Switzerland., Braschler T; Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CMU, Rue Michel-Servet 1, 1211 Genève 4, Switzerland. Electronic address: thomas.braschler@unige.ch.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2022 Apr 01; Vol. 142, pp. 73-84. Date of Electronic Publication: 2022 Jan 31.
DOI: 10.1016/j.actbio.2022.01.050
Abstrakt: Soft tissue reconstruction currently relies on two main approaches, one involving the implantation of external biomaterials and the second one exploiting surgical autologous tissue displacement. While both methods have different advantages and disadvantages, successful long-term solutions for soft tissue repair are still limited. Specifically, volume retention over time and local tissue regeneration are the main challenges in the field. In this study the performance of a recently developed elastic porous injectable (EPI) biomaterial based on crosslinked carboxymethylcellulose is analyzed. Nearly quantitative volumetric stability, with over 90% volume retention at 6 months, is observed, and the pore space of the material is effectively colonized with autologous fibrovascular tissue. A comparative analysis with hyaluronic acid and collagen-based clinical reference materials is also performed. Mechanical stability, evidenced by a low-strain elastic storage modulus (G') approaching 1kPa and a yield strain of several tens of percent, is required for volume retention in-vivo. Macroporosity, along with in-vivo persistence of at least several months, is instead needed for successful host tissue colonization. This study demonstrates the importance of understanding material design criteria and defines the biomaterial requirements for volume retention and tissue colonization in soft tissue regeneration. STATEMENT OF SIGNIFICANCE: We present the design of an elastic, porous, injectable (EPI) scaffold suspension capable of inducing a precisely defined, stable volume of autologous connective tissue in situ. It combines volume stability and vascularized tissue induction capacity known from bulk scaffolds with the ease of injection in shear yielding materials. By comparative study with a series of clinically established biomaterials including a wound healing matrix and dermal fillers, we establish design rules regarding rheological and compressive mechanical properties as well as degradation characteristics that rationally underpin the volume stability and tissue induction in a high-performance biomaterial. These design rules should allow to streamline the development of new colonizable injectables.
Competing Interests: Declaration of Competing Interest A. Béduer and T. Braschler declare financial interests in Volumina-Medical SA, Switzerland. A. Béduer is, M. Martins was an employee of Volumina-Medical SA, Switzerland. Volumina-Medical SA manufactures injectable scaffolds distinct from the ones portrayed here.
(Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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